HXK2/YGL253W Protein Information Help

Standard Name Hxk2p 1
Systematic Name Ygl253wp
Alias Hex1p , Hkbp , Sci2p
ORF Classification Verified
Description Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description HeXoKinase
Experimental Data
Molecules/cell 114000 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 486
Molecular Weight (Da) 53,942
Isoelectric Point (pI) 5.02

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Hxk2p (InterPro)
Physical Interactions There are 42 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000111D88 | P04807
MIPS: YGL253W
NCBI: 1150586 | 1322931 | 14278239 | 157836735 | 171735 | 171739 | 3710 | 3793 | 417162 | 6321184 | NP_011261.1
GenBank/EMBL/DDBJ: DAA07866.1 | M11181 | M14411 | X03483 | X67787 | X94357 | Z72775
External Classifications EC: 2.7.1.1 [Hexokinase]
EC: 2.7.1.4 [Fructokinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MVHLGPKKPQ ARKGSMADVP KELMQQIENF EKIFTVPTET LQAVTKHFIS
      51  ELEKGLSKKG GNIPMIPGWV MDFPTGKESG DFLAIDLGGT NLRVVLVKLG
     101  GDRTFDTTQS KYRLPDAMRT TQNPDELWEF IADSLKAFID EQFPQGISEP
     151  IPLGFTFSFP ASQNKINEGI LQRWTKGFDI PNIENHDVVP MLQKQITKRN
     201  IPIEVVALIN DTTGTLVASY YTDPETKMGV IFGTGVNGAY YDVCSDIEKL
     251  QGKLSDDIPP SAPMAINCEY GSFDNEHVVL PRTKYDITID EESPRPGQQT
     301  FEKMSSGYYL GEILRLALMD MYKQGFIFKN QDLSKFDKPF VMDTSYPARI
     351  EEDPFENLED TDDLFQNEFG INTTVQERKL IRRLSELIGA RAARLSVCGI
     401  AAICQKRGYK TGHIAADGSV YNRYPGFKEK AANALKDIYG WTQTSLDDYP
     451  IKIVPAEDGS GAGAAVIAAL AQKRIAEGKS VGIIGA*

external links for Hxk2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Hxk2p
1) Link, A. and Olson, M.  (1989) Personal Communication, Mortimer Map Edition 10
2) Rodriguez A, et al.  (2001) The hexokinase 2 protein regulates the expression of the GLK1, HXK1 and HXK2 genes of Saccharomyces cerevisiae. Biochem J 355(Pt 3):625-31
3) Bianconi ML  (2003) Calorimetric determination of thermodynamic parameters of reaction reveals different enthalpic compensations of the yeast hexokinase isozymes. J Biol Chem 278(21):18709-13
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Fernandez-Garcia P, et al.  (2012) Phosphorylation of yeast hexokinase 2 regulates its nucleocytoplasmic shuttling. J Biol Chem 287(50):42151-64
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41