RAI1/YGL246C Protein Information

Standard Name	Rai1p 1
Systematic Name	Ygl246cp
ORF Classification	Verified
Description	Nuclear protein with decapping endonuclease activity; targets mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z (1, 2, 3, 4)
Name Description	Rat1p Interacting Protein 1

Experimental Data
Molecules/cell	4030 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	387
Molecular Weight (Da)	44,509
Isoelectric Point (pI)	6.52

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rai1p (InterPro)
Physical Interactions	There are 64 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053188 \| P53063 MIPS: YGL246C NCBI: 1150593 \| 1322917 \| 1723981 \| 51013781 \| 6321191 \| NP_011268.1 \| NM_001181112.1 GenBank/EMBL/DDBJ: DAA07873.1 \| AY693165 \| X94357 \| Z72768
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]

Amino Acid Sequence (or in FASTA format)
1 MGVSANLFVK QRGSTTALKQ PKEIGFYSRT KDEEYLISDD TNLNYYYLPD 51 AELDRKLDLS SGFQKFKDYY KDFEDRCSLR GLLETIESSE RHKGKKINAD 101 IITFRGIARK LISCAFDSPS FNTVDLRIVS FNGQLFIKEV PEAVNAAKAS 151 SATEAGRNIN QDLNVFTGYK FETLATLSNP LQYTPREVIE KRTKRIVSHG 201 DEYISVVRTG VGNCKLILGA EVDCIFDFKE NGRDNLKHYA ELKCTQQVAN 251 ISDTHKFERK LFRTWLQCFL VGIPRIIYGF KDDHYVLKTV EEFSTEEVPV 301 LLKNNNPQVG SACLEAIKWY GLLTEWLLKM IPRDEDPHSQ IRAFKLVFEN 351 NHLRLSEIEE SDEEYSGLID GEHILSNGFK EWRKSLK*

Amino Acid Sequence (or in FASTA format)

       1  MGVSANLFVK QRGSTTALKQ PKEIGFYSRT KDEEYLISDD TNLNYYYLPD
      51  AELDRKLDLS SGFQKFKDYY KDFEDRCSLR GLLETIESSE RHKGKKINAD
     101  IITFRGIARK LISCAFDSPS FNTVDLRIVS FNGQLFIKEV PEAVNAAKAS
     151  SATEAGRNIN QDLNVFTGYK FETLATLSNP LQYTPREVIE KRTKRIVSHG
     201  DEYISVVRTG VGNCKLILGA EVDCIFDFKE NGRDNLKHYA ELKCTQQVAN
     251  ISDTHKFERK LFRTWLQCFL VGIPRIIYGF KDDHYVLKTV EEFSTEEVPV
     301  LLKNNNPQVG SACLEAIKWY GLLTEWLLKM IPRDEDPHSQ IRAFKLVFEN
     351  NHLRLSEIEE SDEEYSGLID GEHILSNGFK EWRKSLK*

external links for Rai1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Rai1p

1)	Xue Y, et al. (2000) Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p. Mol Cell Biol 20(11):4006-15
2)	Sydorskyy Y, et al. (2003) Intersection of the Kap123p-mediated nuclear import and ribosome export pathways. Mol Cell Biol 23(6):2042-54
3)	Jiao X, et al. (2010) Identification of a quality-control mechanism for mRNA 5'-end capping. Nature 467(7315):608-11
4)	Ghosh Dastidar R, et al. (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41