KAP114/YGL241W Protein Information

Standard Name	Kap114p 1
Systematic Name	Ygl241wp
ORF Classification	Verified
Description	Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress (2, 3, 4, 5, 6)
Name Description	KAryoPherin 1

Experimental Data
Molecules/cell	2940 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,004
Molecular Weight (Da)	113,920
Isoelectric Point (pI)	4.38

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Kap114p (InterPro)
Physical Interactions	There are 84 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FA2 \| P53067 MIPS: YGL241W NCBI: 1322907 \| 1723977 \| 6321196 \| 793871 \| NP_011273.1 \| NM_001181107.1 GenBank/EMBL/DDBJ: DAA07878.1 \| Z49149 \| Z72763

Amino Acid Sequence (or in FASTA format)
1 MDINELIIGA QSADKHTREV AETQLLQWCD SDASQVFKAL ANVALQHEAS 51 LESRQFALLS LRKLITMYWS PGFESYRSTS NVEIDVKDFI REVLLKLCLN 101 DNENTKIKNG ASYCIVQISA VDFPDQWPQL LTVIYDAISH QHSLNAMSLL 151 NEIYDDVVSE EMFFEGGIGL ATMEIVFKVL NTETSTLIAK IAALKLLKAC 201 LLQMSSHNEY DEASRKSFVS QCLATSLQIL GQLLTLNFGN VDVISQLKFK 251 SIIYENLVFI KNDFSRKHFS SELQKQFKIM AIQDLENVTH INANVETTES 301 EPLLETVHDC SIYIVEFLTS VCTLQFSVEE MNKIITSLTI LCQLSSETRE 351 IWTSDFNTFV SKETGLAASY NVRDQANEFF TSLPNPQLSL IFKVVSNDIE 401 HSTCNYSTLE SLLYLLQCIL LNDDEITGEN IDQSLQILIK TLENILVSQE 451 IPELILARAI LTIPRVLDKF IDALPDIKPL TSAFLAKSLN LALKSDKELI 501 KSATLIAFTY YCYFAELDSV LGPEVCSETQ EKVIRIINQV SSDAEEDTNG 551 ALMEVLSQVI SYNPKEPHSR KEILQAEFHL VFTISSEDPA NVQVVVQSQE 601 CLEKLLDNIN MDNYKNYIEL CLPSFINVLD SNNANNYRYS PLLSLVLEFI 651 TVFLKKKPND GFLPDEINQY LFEPLAKVLA FSTEDETLQL ATEAFSYLIF 701 NTDTRAMEPR LMDIMKVLER LLSLEVSDSA AMNVGPLVVA IFTRFSKEIQ 751 PLIGRILEAV VVRLIKTQNI STEQNLLSVL CFLTCNDPKQ TVDFLSSFQI 801 DNTDALTLVM RKWIEAFEVI RGEKRIKENI VALSNLFFLN DKRLQKVVVN 851 GNLIPYEGDL IITRSMAKKM PDRYVQVPLY TKIIKLFVSE LSFQSKQPNP 901 EQLITSDIKQ EVVNANKDDD NDDWEDVDDV LDYDKLKEYI DDDVDEEADD 951 DSDDITGLMD VKESVVQLLV RFFKEVASKD VSGFHCIYET LSDSERKVLS 1001 EALL*

Amino Acid Sequence (or in FASTA format)

       1  MDINELIIGA QSADKHTREV AETQLLQWCD SDASQVFKAL ANVALQHEAS
      51  LESRQFALLS LRKLITMYWS PGFESYRSTS NVEIDVKDFI REVLLKLCLN
     101  DNENTKIKNG ASYCIVQISA VDFPDQWPQL LTVIYDAISH QHSLNAMSLL
     151  NEIYDDVVSE EMFFEGGIGL ATMEIVFKVL NTETSTLIAK IAALKLLKAC
     201  LLQMSSHNEY DEASRKSFVS QCLATSLQIL GQLLTLNFGN VDVISQLKFK
     251  SIIYENLVFI KNDFSRKHFS SELQKQFKIM AIQDLENVTH INANVETTES
     301  EPLLETVHDC SIYIVEFLTS VCTLQFSVEE MNKIITSLTI LCQLSSETRE
     351  IWTSDFNTFV SKETGLAASY NVRDQANEFF TSLPNPQLSL IFKVVSNDIE
     401  HSTCNYSTLE SLLYLLQCIL LNDDEITGEN IDQSLQILIK TLENILVSQE
     451  IPELILARAI LTIPRVLDKF IDALPDIKPL TSAFLAKSLN LALKSDKELI
     501  KSATLIAFTY YCYFAELDSV LGPEVCSETQ EKVIRIINQV SSDAEEDTNG
     551  ALMEVLSQVI SYNPKEPHSR KEILQAEFHL VFTISSEDPA NVQVVVQSQE
     601  CLEKLLDNIN MDNYKNYIEL CLPSFINVLD SNNANNYRYS PLLSLVLEFI
     651  TVFLKKKPND GFLPDEINQY LFEPLAKVLA FSTEDETLQL ATEAFSYLIF
     701  NTDTRAMEPR LMDIMKVLER LLSLEVSDSA AMNVGPLVVA IFTRFSKEIQ
     751  PLIGRILEAV VVRLIKTQNI STEQNLLSVL CFLTCNDPKQ TVDFLSSFQI
     801  DNTDALTLVM RKWIEAFEVI RGEKRIKENI VALSNLFFLN DKRLQKVVVN
     851  GNLIPYEGDL IITRSMAKKM PDRYVQVPLY TKIIKLFVSE LSFQSKQPNP
     901  EQLITSDIKQ EVVNANKDDD NDDWEDVDDV LDYDKLKEYI DDDVDEEADD
     951  DSDDITGLMD VKESVVQLLV RFFKEVASKD VSGFHCIYET LSDSERKVLS
    1001  EALL*

external links for Kap114p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Kap114p

1)	Pemberton LF, et al. (1999) Nuclear import of the TATA-binding protein: mediation by the karyopherin Kap114p and a possible mechanism for intranuclear targeting. J Cell Biol 145(7):1407-17
2)	Morehouse H, et al. (1999) The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein. Proc Natl Acad Sci U S A 96(22):12542-7
3)	Mosammaparast N, et al. (2002) A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B. EMBO J 21(23):6527-38
4)	Hodges JL, et al. (2005) Nuclear import of TFIIB is mediated by Kap114p, a karyopherin with multiple cargo-binding domains. Mol Biol Cell 16(7):3200-10
5)	Rothenbusch U, et al. (2012) Sumoylation regulates Kap114-mediated nuclear transport. EMBO J 31(11):2461-72
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41