DOC1/YGL240W Protein Information

Standard Name	Doc1p
Systematic Name	Ygl240wp
Alias	Apc10p
ORF Classification	Verified
Description	Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain (1, 2, 3, 4, 5)
Name Description	Destruction Of Cyclin B 1

Experimental Data
Molecules/cell	1360 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	250
Molecular Weight (Da)	28,776
Isoelectric Point (pI)	5.54

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Doc1p (InterPro)
Physical Interactions	There are 74 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001D4780 \| P53068 MIPS: YGL240W NCBI: 1322905 \| 1945308 \| 20149881 \| 20149882 \| 37362650 \| 55584167 \| 793873 \| NP_011274.2 GenBank/EMBL/DDBJ: DAA07879.1 \| Z49149 \| Z72761 \| Z72762

Amino Acid Sequence (or in FASTA format)
1 MDPIGINKVL DHLAPSELIK PVKSCHNKPS VLVLDDRIVD AATKDLYVNG 51 FQEEIQYQNP TPENLQHMFH QGIEILDSAR MINVTHLALW KPSSFKLGNP 101 VDFALDDNYD TFWQSDGGQP HQLDIMFSKR MDICVMAIFF SMIADESYAP 151 SLVKVYAGHS PSDARFYKML EVRNVNGWVA LRFLDNREDD QLLKCQFIRL 201 LFPVNHENGK DTHLRGIRLY VPSNEPHQDT HEWAQTLPET NNVFQDAILR 251 *

Amino Acid Sequence (or in FASTA format)

       1  MDPIGINKVL DHLAPSELIK PVKSCHNKPS VLVLDDRIVD AATKDLYVNG
      51  FQEEIQYQNP TPENLQHMFH QGIEILDSAR MINVTHLALW KPSSFKLGNP
     101  VDFALDDNYD TFWQSDGGQP HQLDIMFSKR MDICVMAIFF SMIADESYAP
     151  SLVKVYAGHS PSDARFYKML EVRNVNGWVA LRFLDNREDD QLLKCQFIRL
     201  LFPVNHENGK DTHLRGIRLY VPSNEPHQDT HEWAQTLPET NNVFQDAILR
     251  *

external links for Doc1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Doc1p

1)	Hwang LH and Murray AW (1997) A novel yeast screen for mitotic arrest mutants identifies DOC1, a new gene involved in cyclin proteolysis. Mol Biol Cell 8(10):1877-87
2)	Grossberger R, et al. (1999) Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. J Biol Chem 274(20):14500-7
3)	Au SW, et al. (2002) Implications for the ubiquitination reaction of the anaphase-promoting complex from the crystal structure of the Doc1/Apc10 subunit. J Mol Biol 316(4):955-68
4)	Carroll CW and Morgan DO (2002) The Doc1 subunit is a processivity factor for the anaphase-promoting complex. Nat Cell Biol 4(11):880-7
5)	Passmore LA, et al. (2003) Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J 22(4):786-96
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41