VRG4/YGL225W Protein Information

Standard Name	Vrg4p 1, 2
Systematic Name	Ygl225wp
Alias	Van2p 3 , Gog5p , Ldb3p , Vig4p 4
ORF Classification	Verified
Description	Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi; VRG4 has a paralog, HVG1, that arose from the whole genome duplication (1, 2, 5, 6)
Name Description	Vandate Resistance Glycosylation 1

Experimental Data
Molecules/cell	31900 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	337
Molecular Weight (Da)	37,018
Isoelectric Point (pI)	10.08

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Vrg4p (InterPro)
Transmembrane Domains	There are 9 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 13 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012B8DF \| P40107 MIPS: YGL225W NCBI: 1322877 \| 499894 \| 603046 \| 6321213 \| 729611 \| NP_011290.1 GenBank/EMBL/DDBJ: DAA07894.1 \| L33915 \| U15599 \| Z72747
External Classifications	TC: 2.A.7.13 [The GDP-Mannose:GMP Antiporter (GMA) Family]

Amino Acid Sequence (or in FASTA format)
1 MSELKTGHAG HNPWASVANS GPISILSYCG SSILMTVTNK FVVNLKDFNM 51 NFVMLFVQSL VCTITLIILR ILGYAKFRSL NKTDAKNWFP ISFLLVLMIY 101 TSSKALQYLA VPIYTIFKNL TIILIAYGEV LFFGGSVTSM ELSSFLLMVL 151 SSVVATWGDQ QAVAAKAASL AEGAAGAVAS FNPGYFWMFT NCITSALFVL 201 IMRKRIKLTN FKDFDTMFYN NVLALPILLL FSFCVEDWSS VNLTNNFSND 251 SLTAMIISGV ASVGISYCSG WCVRVTSSTT YSMVGALNKL PIALSGLIFF 301 DAPRNFLSIL SIFIGFLSGI IYAVAKQKKQ QAQPLRK*

Amino Acid Sequence (or in FASTA format)

       1  MSELKTGHAG HNPWASVANS GPISILSYCG SSILMTVTNK FVVNLKDFNM
      51  NFVMLFVQSL VCTITLIILR ILGYAKFRSL NKTDAKNWFP ISFLLVLMIY
     101  TSSKALQYLA VPIYTIFKNL TIILIAYGEV LFFGGSVTSM ELSSFLLMVL
     151  SSVVATWGDQ QAVAAKAASL AEGAAGAVAS FNPGYFWMFT NCITSALFVL
     201  IMRKRIKLTN FKDFDTMFYN NVLALPILLL FSFCVEDWSS VNLTNNFSND
     251  SLTAMIISGV ASVGISYCSG WCVRVTSSTT YSMVGALNKL PIALSGLIFF
     301  DAPRNFLSIL SIFIGFLSGI IYAVAKQKKQ QAQPLRK*

external links for Vrg4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	TCDB
YOGY	DIP	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Vrg4p

1)	Ballou L, et al. (1991) Vanadate-resistant yeast mutants are defective in protein glycosylation. Proc Natl Acad Sci U S A 88(8):3209-12
2)	Poster JB and Dean N (1996) The yeast VRG4 gene is required for normal Golgi functions and defines a new family of related genes. J Biol Chem 271(7):3837-45
3)	Kanik-Ennulat C and Neff N (1990) Vanadate-resistant mutants of Saccharomyces cerevisiae show alterations in protein phosphorylation and growth control. Mol Cell Biol 10(3):898-909
4)	Abe M, et al. (1999) Molecular characterization of Vig4/Vrg4 GDP-mannose transporter of the yeast Saccharomyces cerevisiae. FEBS Lett 458(3):309-12
5)	Dean N, et al. (1997) The VRG4 gene is required for GDP-mannose transport into the lumen of the Golgi in the yeast, Saccharomyces cerevisiae. J Biol Chem 272(50):31908-14
6)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41