SKI8/YGL213C Protein Information

Standard Name	Ski8p 1
Systematic Name	Ygl213cp
Alias	Rec103p 2
ORF Classification	Verified
Description	Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype (1, 3, 4, 5, 6, 7)
Name Description	SuperKIller 1

Experimental Data
Molecules/cell	1710 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	397
Molecular Weight (Da)	44,231
Isoelectric Point (pI)	5.34

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ski8p (InterPro)
Physical Interactions	There are 56 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000036C4F \| Q02793 MIPS: YGL213C NCBI: 1322857 \| 172614 \| 401086 \| 49259107 \| 58176701 \| 6321225 \| NP_011302.1 GenBank/EMBL/DDBJ: DAA07903.1 \| M96058 \| Z72736

Amino Acid Sequence (or in FASTA format)
1 MSKVFIATAN AGKAHDADIF SVSACNSFTV SCSGDGYLKV WDNKLLDNEN 51 PKDKSYSHFV HKSGLHHVDV LQAIERDAFE LCLVATTSFS GDLLFYRITR 101 EDETKKVIFE KLDLLDSDMK KHSFWALKWG ASNDRLLSHR LVATDVKGTT 151 YIWKFHPFAD ESNSLTLNWS PTLELQGTVE SPMTPSQFAT SVDISERGLI 201 ATGFNNGTVQ ISELSTLRPL YNFESQHSMI NNSNSIRSVK FSPQGSLLAI 251 AHDSNSFGCI TLYETEFGER IGSLSVPTHS SQASLGEFAH SSWVMSLSFN 301 DSGETLCSAG WDGKLRFWDV KTKERITTLN MHCDDIEIEE DILAVDEHGD 351 SLAEPGVFDV KFLKKGWRSG MGADLNESLC CVCLDRSIRW FREAGGK*

Amino Acid Sequence (or in FASTA format)

       1  MSKVFIATAN AGKAHDADIF SVSACNSFTV SCSGDGYLKV WDNKLLDNEN
      51  PKDKSYSHFV HKSGLHHVDV LQAIERDAFE LCLVATTSFS GDLLFYRITR
     101  EDETKKVIFE KLDLLDSDMK KHSFWALKWG ASNDRLLSHR LVATDVKGTT
     151  YIWKFHPFAD ESNSLTLNWS PTLELQGTVE SPMTPSQFAT SVDISERGLI
     201  ATGFNNGTVQ ISELSTLRPL YNFESQHSMI NNSNSIRSVK FSPQGSLLAI
     251  AHDSNSFGCI TLYETEFGER IGSLSVPTHS SQASLGEFAH SSWVMSLSFN
     301  DSGETLCSAG WDGKLRFWDV KTKERITTLN MHCDDIEIEE DILAVDEHGD
     351  SLAEPGVFDV KFLKKGWRSG MGADLNESLC CVCLDRSIRW FREAGGK*

external links for Ski8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ski8p

1)	Ridley SP, et al. (1984) Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN. Mol Cell Biol 4(4):761-70
2)	Gardiner JM, et al. (1997) Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast. Genetics 146(4):1265-74
3)	Brown JT, et al. (2000) The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo. RNA 6(3):449-57
4)	Anderson JS and Parker RP (1998) The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex. EMBO J 17(5):1497-506
5)	Arora C, et al. (2004) Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism. Mol Cell 13(4):549-59
6)	Cheng Z, et al. (2004) Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination. Protein Sci 13(10):2673-84
7)	Madrona AY and Wilson DK (2004) The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure. Protein Sci 13(6):1557-65
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41