NCS6/YGL211W Protein Information

Standard Name	Ncs6p
Systematic Name	Ygl211wp
Alias	Ygl210w-ap , Tuc1p 1
ORF Classification	Verified
Description	Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae (1, 2, 3)
Name Description	Needs Cla4 to Survive 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	359
Molecular Weight (Da)	39,987
Isoelectric Point (pI)	8.97

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ncs6p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00003FEC72 \| P53088 MIPS: YGL211W NCBI: 115502422 \| 1322852 \| 1655726 \| 2507513 \| 50593215 \| NP_011304.2 GenBank/EMBL/DDBJ: DAA07905.1 \| U33754 \| Z72733
External Classifications	EC: 2.7.7.- [Transferases transferring phosphorous-containing groups nucleotidyltransferases]

Amino Acid Sequence (or in FASTA format)
1 MSFTAPSDPV NKPTKVKVSQ LCELCHSRKA LIRRPKNLSK LCKQCFCLVF 51 ETEIHNTIVA NNLFQRGEKV AVGASGGKDS TVLAHMLKLL NDRYDYGIEI 101 VLLSIDEGII GYRDDSLATV KRNQQQYGLP LEIFSFKDLY DWTMDEIVSV 151 AGIRNSCTYC GVFRRQSLDR GAAKLGISHV VTGHNADDMA ETVLMNILRG 201 DVARLEKSTA IITQSSGSPI KRSKPFKYSY QKEIVLYAHY MKLDYFSTEC 251 TYAPEAFRGT AREYMKNLEA VRPSCIIDII QSGENLALKA KKSNAGKRVV 301 KFVDGNRCAR CGYLSSNNIC KACMLLEGLE KSRAQVAIEN DTSADGAALK 351 LRALEKLSF*

Amino Acid Sequence (or in FASTA format)

       1  MSFTAPSDPV NKPTKVKVSQ LCELCHSRKA LIRRPKNLSK LCKQCFCLVF
      51  ETEIHNTIVA NNLFQRGEKV AVGASGGKDS TVLAHMLKLL NDRYDYGIEI
     101  VLLSIDEGII GYRDDSLATV KRNQQQYGLP LEIFSFKDLY DWTMDEIVSV
     151  AGIRNSCTYC GVFRRQSLDR GAAKLGISHV VTGHNADDMA ETVLMNILRG
     201  DVARLEKSTA IITQSSGSPI KRSKPFKYSY QKEIVLYAHY MKLDYFSTEC
     251  TYAPEAFRGT AREYMKNLEA VRPSCIIDII QSGENLALKA KKSNAGKRVV
     301  KFVDGNRCAR CGYLSSNNIC KACMLLEGLE KSRAQVAIEN DTSADGAALK
     351  LRALEKLSF*

external links for Ncs6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Ncs6p

1)	Bjork GR, et al. (2007) A conserved modified wobble nucleoside (mcm5s2U) in lysyl-tRNA is required for viability in yeast. RNA 13(8):1245-55
2)	Goehring AS, et al. (2003) Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast. Mol Biol Cell 14(11):4329-41
3)	Kushner DB, et al. (2003) Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus. Proc Natl Acad Sci U S A 100(26):15764-9
4)	Goehring AS, et al. (2003) Synthetic lethal analysis implicates Ste20p, a p21-activated potein kinase, in polarisome activation. Mol Biol Cell 14(4):1501-16