SIP2/YGL208W Protein Information Help

Standard Name Sip2p 1
Systematic Name Ygl208wp
Alias Spm2p
ORF Classification Verified
Description One of three beta subunits of the Snf1 kinase complex; involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane; SIP2 has a paralog, GAL83, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description SNF1-Interacting Protein 7
Experimental Data
Molecules/cell 300 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 415
Molecular Weight (Da) 46,404
Isoelectric Point (pI) 5.43

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sip2p (InterPro)
Physical Interactions There are 47 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052F21 | P34164
MIPS: YGL208W
NCBI: 1322846 | 158430319 | 158430322 | 287915 | 356624534 | 356624590 | 356624593 | 3645897 | 464800 | 468428 | 6321230 | NP_011307.1
GenBank/EMBL/DDBJ: DAA07908.1 | L31592 | U33754 | Z14128 | Z72730
Amino Acid Sequence (or in FASTA format)
 
       1  MGTTTSHPAQ KKQTTKKCRA PIMSDVREKP SNAQGCEPQE MDAVSKKVTE
      51  LSLNKCSDSQ DAGQPSREGS ITKKKSTLLL RDEDEPTMPK LSVMETAVDT
     101  DSGSSSTSDD EEGDIIAQTT EPKQDASPDD DRSGHSSPRE EGQQQIRAKE
     151  ASGGPSEIKS SLMVPVEIRW QQGGSKVYVT GSFTKWRKMI GLIPDSDNNG
     201  SFHVKLRLLP GTHRFRFIVD NELRVSDFLP TATDQMGNFV NYIEVRQPEK
     251  NPTNEKIRSK EADSMRPPTS DRSSIALQIG KDPDDFGDGY TRFHEDLSPR
     301  PPLEYTTDIP AVFTDPSVME RYYYTLDRQQ SNTDTSWLTP PQLPPQLENV
     351  ILNKYYATQD QFNENNSGAL PIPNHVVLNH LVTSSIKHNT LCVASIVRYK
     401  QKYVTQILYT PIESS*

external links for Sip2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Sip2p
1) Yang X, et al.  (1994) A family of proteins containing a conserved domain that mediates interaction with the yeast SNF1 protein kinase complex. EMBO J 13(24):5878-86
2) Ashrafi K, et al.  (2000) Sip2p and its partner snf1p kinase affect aging in S. cerevisiae. Genes Dev 14(15):1872-85
3) Schmidt MC and McCartney RR  (2000) beta-subunits of Snf1 kinase are required for kinase function and substrate definition. EMBO J 19(18):4936-43
4) Vincent O, et al.  (2001) Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism. Genes Dev 15(9):1104-14
5) Lin SS, et al.  (2003) Sip2, an N-myristoylated beta subunit of Snf1 kinase, regulates aging in Saccharomyces cerevisiae by affecting cellular histone kinase activity, recombination at rDNA loci, and silencing. J Biol Chem 278(15):13390-7
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Yang X, et al.  (1992) A protein kinase substrate identified by the two-hybrid system. Science 257(5070):680-2
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41