MCM6/YGL201C Protein Information Help

Standard Name Mcm6p 1
Systematic Name Ygl201cp
ORF Classification Verified
Description Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p (2, 3, 4, 5)
Name Description MiniChromosome Maintenance 6
Experimental Data
Molecules/cell 13400 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,017
Molecular Weight (Da) 112,977
Isoelectric Point (pI) 4.88

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mcm6p (InterPro)
Physical Interactions There are 116 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000018CCFF | P53091
MIPS: YGL201C
NCBI: 1322832 | 1723953 | 29725889 | 2988342 | 308153465 | 41629691 | NP_011314.2 | NM_001181066.1
GenBank/EMBL/DDBJ: AAO89010.1 | CAA73947.1 | CAA96913.1 | DAA07914.1 | AY258324 | Y13624 | Z72723
External Classifications EC: 3.6.4.12 [DNA helicase]
Amino Acid Sequence (or in FASTA format)
       1  MSSPFPADTP SSNRPSNSSP PPSSIGAGFG SSSGLDSQIG SRLHFPSSSQ
      51  PHVSNSQTGP FVNDSTQFSS QRLQTDGSAT NDMEGNEPAR SFKSRALNHV
     101  KKVDDVTGEK VREAFEQFLE DFSVQSTDTG EVEKVYRAQI EFMKIYDLNT
     151  IYIDYQHLSM RENGALAMAI SEQYYRFLPF LQKGLRRVVR KYAPELLNTS
     201  DSLKRSEGDE GQADEDEQQD DDMNGSSLPR DSGSSAAPGN GTSAMATRSI
     251  TTSTSPEQTE RVFQISFFNL PTVHRIRDIR SEKIGSLLSI SGTVTRTSEV
     301  RPELYKASFT CDMCRAIVDN VEQSFKYTEP TFCPNPSCEN RAFWTLNVTR
     351  SRFLDWQKVR IQENANEIPT GSMPRTLDVI LRGDSVERAK PGDRCKFTGV
     401  EIVVPDVTQL GLPGVKPSST LDTRGISKTT EGLNSGVTGL RSLGVRDLTY
     451  KISFLACHVI SIGSNIGASS PDANSNNRET ELQMAANLQA NNVYQDNERD
     501  QEVFLNSLSS DEINELKEMV KDEHIYDKLV RSIAPAVFGH EAVKKGILLQ
     551  MLGGVHKSTV EGIKLRGDIN ICVVGDPSTS KSQFLKYVVG FAPRSVYTSG
     601  KASSAAGLTA AVVRDEEGGD YTIEAGALML ADNGICCIDE FDKMDISDQV
     651  AIHEAMEQQT ISIAKAGIHA TLNARTSILA AANPVGGRYN RKLSLRGNLN
     701  MTAPIMSRFD LFFVILDDCN EKIDTELASH IVDLHMKRDE AIEPPFSAEQ
     751  LRRYIKYART FKPILTKEAR SYLVEKYKEL RKDDAQGFSR SSYRITVRQL
     801  ESMIRLSEAI ARANCVDEIT PSFIAEAYDL LRQSIIRVDV DDVEMDEEFD
     851  NIESQSHAAS GNNDDNDDGT GSGVITSEPP ADIEEGQSEA TARPGTSEKK
     901  KTTVTYDKYV SMMNMIVRKI AEVDREGAEE LTAVDIVDWY LLQKENDLGS
     951  LAEYWEERRL AFKVIKRLVK DRILMEIHGT RHNLRDLENE ENENNKTVYV
    1001  IHPNCEVLDQ LEPQDSS*                                   

external links for Mcm6p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) CYC2008 (complexes) MIPS YeastGFP
Candida (CGD) Complexome Pfam domains
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mcm6p
1) Tye BK  (1999) Minichromosome maintenance as a genetic assay for defects in DNA replication. Methods 18(3):329-34
2) Homesley L, et al.  (2000) Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins. Genes Dev 14(8):913-26
3) You Z, et al.  (1999) Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity. Mol Cell Biol 19(12):8003-15
4) Ma X, et al.  (2010) The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function. BMC Biochem 11():37
5) Bochman ML and Schwacha A  (2008) The Mcm2-7 complex has in vitro helicase activity. Mol Cell 31(2):287-93
6) Maine GT, et al.  (1984) Mutants of S. cerevisiae defective in the maintenance of minichromosomes. Genetics 106(3):365-85
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41