IME4/YGL192W Protein Information Help

Standard Name Ime4p 1
Systematic Name Ygl192wp
Alias Spo8p
ORF Classification Verified
Description mRNA N6-adenosine methyltransferase required for entry into meiosis; mediates N6-adenosine methylation of bulk mRNA during the induction of sporulation which includes the meiotic regulators IME1, IME2 and IME4 itself; repressed in haploids via production of antisense IME4 transcripts; transcribed in diploid cells where antisense transcription is repressed; orthologous to human METTL3 (MT-A70) (1, 2, 3, 4, 5)
Name Description Inducer of MEiosis 1, 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 600
Molecular Weight (Da) 69,395
Isoelectric Point (pI) 7.99

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ime4p (InterPro)
Physical Interactions There are 10 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000135E5D | P41833
MIPS: YGL192W
NCBI: 1174426 | 1177636 | 1322815 | 398364297 | 471168 | 945046 | NP_011323.3
GenBank/EMBL/DDBJ: DAA07923.1 | D23721 | U30859 | X91837 | Z72714
External Classifications EC: 2.1.1.62 [mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase]
Amino Acid Sequence (or in FASTA format)
 
       1  MINDKLVHFL IQNYDDILRA PLSGQLKDVY SLYISGGYDD EMQKLRNDKD
      51  EVLQFEQFWN DLQDIIFATP QSIQFDQNLL VADRPEKIVY LDVFSLKILY
     101  NKFHAFYYTL KSSSSSCEEK VSSLTTKPEA DSEKDQLLGR LLGVLNWDVN
     151  VSNQGLPREQ LSNRLQNLLR EKPSSFQLAK ERAKYTTEVI EYIPICSDYS
     201  HASLLSTSVY IVNNKIVSLQ WSKISACQEN HPGLIECIQS KIHFIPNIKP
     251  QTDISLGDCS YLDTCHKLNT CRYIHYLQYI PSCLQERADR ETASENKRIR
     301  SNVSIPFYTL GNCSAHCIKK ALPAQWIRCD VRKFDFRVLG KFSVVIADPA
     351  WNIHMNLPYG TCNDIELLGL PLHELQDEGI IFLWVTGRAI ELGKESLNNW
     401  GYNVINEVSW IKTNQLGRTI VTGRTGHWLN HSKEHLLVGL KGNPKWINKH
     451  IDVDLIVSMT RETSRKPDEL YGIAERLAGT HARKLEIFGR DHNTRPGWFT
     501  IGNQLTGNCI YEMDVERKYQ EFMKSKTGTS HTGTKKIDKK QPSKLQQQHQ
     551  QQYWNNMDMG SGKYYAEAKQ NPMNQKHTPF ESKQQQKQQF QTLNNLYFAQ
     601  *

external links for Ime4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ime4p
1) Shah JC and Clancy MJ  (1992) IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae. Mol Cell Biol 12(3):1078-86
2) Clancy MJ, et al.  (2002) Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene. Nucleic Acids Res 30(20):4509-18
3) Hongay CF, et al.  (2006) Antisense transcription controls cell fate in Saccharomyces cerevisiae. Cell 127(4):735-45
4) Bodi Z, et al.  (2010) Yeast targets for mRNA methylation. Nucleic Acids Res 38(16):5327-35
5) Dominissini D, et al.  (2012) Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature 485(7397):201-6
6) Kassir Y, et al.  (1988) IME1, a positive regulator gene of meiosis in S. cerevisiae. Cell 52(6):853-62