CDC55/YGL190C Protein Information Help

Standard Name Cdc55p 1
Systematic Name Ygl190cp
ORF Classification Verified
Description Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion (2, 3, 4, 5, 6, 7)
Name Description Cell Division Cycle
Experimental Data
Molecules/cell 8600 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 526
Molecular Weight (Da) 59,662
Isoelectric Point (pI) 6.36

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Cdc55p (InterPro)
Physical Interactions There are 119 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000530B4 | Q00362
MIPS: YGL190C
NCBI: 1143558 | 1177638 | 1322811 | 1702999 | 171195 | 6321248 | NP_011325.1
GenBank/EMBL/DDBJ: DAA07925.1 | M72716 | X91489 | X91837 | Z72712
Amino Acid Sequence (or in FASTA format)
 
       1  MAQNNFDFKF SQCFGDKADI VVTEADLITA VEFDYTGNYL ATGDKGGRVV
      51  LFERSNSRHC EYKFLTEFQS HDAEFDYLKS LEIEEKINEI KWLRPTQRSH
     101  FLLSTNDKTI KLWKVYEKNI KLVSQNNLTE GVTFAKKGKP DNHNSRGGSV
     151  RAVLSLQSLK LPQLSQHDKI IAATPKRIYS NAHTYHINSI SLNSDQETFL
     201  SADDLRINLW NLDIPDQSFN IVDIKPTNME ELTEVITSAE FHPQECNLFM
     251  YSSSKGTIKL CDMRQNSLCD NKTKTFEEYL DPINHNFFTE ITSSISDIKF
     301  SPNGRYIASR DYLTVKIWDV NMDNKPLKTI NIHEQLKERL SDTYENDAIF
     351  DKFEVNFSGD SSSVMTGSYN NNFMIYPNVV TSGDNDNGIV KTFDEHNAPN
     401  SNSNKNIHNS IQNKDSSSSG NSHKRRSNGR NTGMVGSSNS SRSSIAGGEG
     451  ANSEDSGTEM NEIVLQADKT AFRNKRYGSL AQRSARNKDW GDDIDFKKNI
     501  LHFSWHPREN SIAVAATNNL FIFSAL*

external links for Cdc55p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Cdc55p
1) Healy AM, et al.  (1991) CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase. Mol Cell Biol 11(11):5767-80
2) Jiang Y and Broach JR  (1999) Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast. EMBO J 18(10):2782-92
3) Wang Y and Burke DJ  (1997) Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae. Mol Cell Biol 17(2):620-6
4) Gentry MS and Hallberg RL  (2002) Localization of Saccharomyces cerevisiae protein phosphatase 2A subunits throughout mitotic cell cycle. Mol Biol Cell 13(10):3477-92
5) Queralt E, et al.  (2006) Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast. Cell 125(4):719-32
6) Rossio V and Yoshida S  (2011) Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast. J Cell Biol 193(3):445-54
7) Bizzari F and Marston AL  (2011) Cdc55 coordinates spindle assembly and chromosome disjunction during meiosis. J Cell Biol 193(7):1213-28
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41