ATG1/YGL180W Protein Information

Standard Name	Atg1p 1
Systematic Name	Ygl180wp
Alias	Aut3p 2 , Cvt10p 3 , Apg1p 4
ORF Classification	Verified
Description	Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p (5, 6, 7, 8, 9)
Name Description	AuTophaGy related 1

Experimental Data
Molecules/cell	1070 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	897
Molecular Weight (Da)	101,716
Isoelectric Point (pI)	6.61

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Atg1p (InterPro)
Physical Interactions	There are 269 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000125C5E \| P53104 MIPS: YGL180W NCBI: 1143567 \| 1322791 \| 1730043 \| 2257625 \| 6321258 \| NP_011335.1 GenBank/EMBL/DDBJ: DAA07934.1 \| D29991 \| X91489 \| Z72702
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MGDIKNKDHT TSVNHNLMAS AGNYTAEKEI GKGSFATVYR GHLTSDKSQH 51 VAIKEVSRAK LKNKKLLENL EIEIAILKKI KHPHIVGLID CERTSTDFYL 101 IMEYCALGDL TFLLKRRKEL MENHPLLRTV FEKYPPPSEN HNGLHRAFVL 151 SYLQQLASAL KFLRSKNLVH RDIKPQNLLL STPLIGYHDS KSFHELGFVG 201 IYNLPILKIA DFGFARFLPN TSLAETLCGS PLYMAPEILN YQKYNAKADL 251 WSVGTVVFEM CCGTPPFRAS NHLELFKKIK RANDVITFPS YCNIEPELKE 301 LICSLLTFDP AQRIGFEEFF ANKVVNEDLS SYELEDDLPE LESKSKGIVE 351 SNMFVSEYLS KQPKSPNSNL AGHQSMADNP AELSDALKNS NILTAPAVKT 401 DHTQAVDKKA SNNKYHNSLV SDRSFEREYV VVEKKSVEVN SLADEVAQAG 451 FNPNPIKHPT STQNQNVLLN EQFSPNNQQY FQNQGENPRL LRATSSSSGG 501 SDGSRRPSLV DRRLSISSLN PSNALSRALG IASTRLFGGA NQQQQQQQIT 551 SSPPYSQTLL NSQLFHELTE NIILRIDHLQ HPETLKLDNT NIVSILESLA 601 AKAFVVYSYA EVKFSQIVPL STTLKGMANF ENRRSMDSNA IAEEQDSDDA 651 EEEDETLKKY KEDCLSTKTF GKGRTLSATS QLSATFNKLP RSEMILLCNE 701 AIVLYMKALS ILSKSMQVTS NWWYESQEKS CSLRVNVLVQ WLREKFNECL 751 EKADFLRLKI NDLRFKHASE VAENQTLEEK GSSEEPVYLE KLLYDRALEI 801 SKMAAHMELK GENLYNCELA YATSLWMLET SLDDDDFTNA YGDYPFKTNI 851 HLKSNDVEDK EKYHSVLDEN DRIIIRKYID SIANRLKILR QKMNHQN*

Amino Acid Sequence (or in FASTA format)

       1  MGDIKNKDHT TSVNHNLMAS AGNYTAEKEI GKGSFATVYR GHLTSDKSQH
      51  VAIKEVSRAK LKNKKLLENL EIEIAILKKI KHPHIVGLID CERTSTDFYL
     101  IMEYCALGDL TFLLKRRKEL MENHPLLRTV FEKYPPPSEN HNGLHRAFVL
     151  SYLQQLASAL KFLRSKNLVH RDIKPQNLLL STPLIGYHDS KSFHELGFVG
     201  IYNLPILKIA DFGFARFLPN TSLAETLCGS PLYMAPEILN YQKYNAKADL
     251  WSVGTVVFEM CCGTPPFRAS NHLELFKKIK RANDVITFPS YCNIEPELKE
     301  LICSLLTFDP AQRIGFEEFF ANKVVNEDLS SYELEDDLPE LESKSKGIVE
     351  SNMFVSEYLS KQPKSPNSNL AGHQSMADNP AELSDALKNS NILTAPAVKT
     401  DHTQAVDKKA SNNKYHNSLV SDRSFEREYV VVEKKSVEVN SLADEVAQAG
     451  FNPNPIKHPT STQNQNVLLN EQFSPNNQQY FQNQGENPRL LRATSSSSGG
     501  SDGSRRPSLV DRRLSISSLN PSNALSRALG IASTRLFGGA NQQQQQQQIT
     551  SSPPYSQTLL NSQLFHELTE NIILRIDHLQ HPETLKLDNT NIVSILESLA
     601  AKAFVVYSYA EVKFSQIVPL STTLKGMANF ENRRSMDSNA IAEEQDSDDA
     651  EEEDETLKKY KEDCLSTKTF GKGRTLSATS QLSATFNKLP RSEMILLCNE
     701  AIVLYMKALS ILSKSMQVTS NWWYESQEKS CSLRVNVLVQ WLREKFNECL
     751  EKADFLRLKI NDLRFKHASE VAENQTLEEK GSSEEPVYLE KLLYDRALEI
     801  SKMAAHMELK GENLYNCELA YATSLWMLET SLDDDDFTNA YGDYPFKTNI
     851  HLKSNDVEDK EKYHSVLDEN DRIIIRKYID SIANRLKILR QKMNHQN*

external links for Atg1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Atg1p

1)	Klionsky DJ, et al. (2003) A unified nomenclature for yeast autophagy-related genes. Dev Cell 5(4):539-45
2)	Thumm M, et al. (1994) Isolation of autophagocytosis mutants of Saccharomyces cerevisiae. FEBS Lett 349(2):275-80
3)	Harding TM, et al. (1996) Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway. J Biol Chem 271(30):17621-4
4)	Tsukada M and Ohsumi Y (1993) Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Lett 333(1-2):169-74
5)	Straub M, et al. (1997) AUT3, a serine/threonine kinase gene, is essential for autophagocytosis in Saccharomyces cerevisiae. J Bacteriol 179(12):3875-83
6)	Matsuura A, et al. (1997) Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae. Gene 192(2):245-50
7)	Abeliovich H, et al. (2003) Chemical genetic analysis of Apg1 reveals a non-kinase role in the induction of autophagy. Mol Biol Cell 14(2):477-90
8)	Kabeya Y, et al. (2005) Atg17 functions in cooperation with Atg1 and Atg13 in yeast autophagy. Mol Biol Cell 16(5):2544-53
9)	Cheong H, et al. (2008) The Atg1 Kinase Complex Is Involved in the Regulation of Protein Recruitment to Initiate Sequestering Vesicle Formation for Nonspecific Autophagy in Saccharomyces cerevisiae. Mol Biol Cell 19(2):668-81
10)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41