TOS3/YGL179C Protein Information Help

Standard Name Tos3p
Systematic Name Ygl179cp
ORF Classification Verified
Description Protein kinase; related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; TOS3 has a paralog, SAK1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description Target Of Sbf
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 560
Molecular Weight (Da) 62,090
Isoelectric Point (pI) 7.41

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tos3p (InterPro)
Physical Interactions There are 87 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012DDCE | P43637
MIPS: YGL179C
NCBI: 1170647 | 1322789 | 398364315 | 794144 | NP_011336.3
GenBank/EMBL/DDBJ: DAA07935.1 | X83690 | Z72701
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MVLLKEPVQP LPRSSLLYNN ASNSSSRIKE TRKVKLLYNP LTKRQILNNF
      51  EILATLGNGQ YGKVKLARDL GTGALVAIKI LNRFEKRSGY SLQLKVENPR
     101  VNQEIEVMKR CHHENVVELY EILNDPESTK VYLVLEYCSR GPVKWCPENK
     151  MEIKAVGPSI LTFQQSRKVV LDVVSGLEYL HSQGITHRDI KPSNLLISSN
     201  GTVKISDFGV AMSTATGSTN IQSSHEQLLK SRALGTPAFF APELCSTEKE
     251  YSCSSAIDIW SLGVTIYCLL FGKLPFNANS GLELFDSIIN KPLEFPSYEE
     301  MLNGATSGIT MEEYTDAKDL LKKLLQKDPD KRIKLADIKV HPFMCHYGKS
     351  DAASVLTNLE TFHELKVSPP SSCKRVELVS LPVNSSFASL DSVYMENFDH
     401  NNLRTGADRN STYSPSIYDA NTLSPSAYHN IGSRESSYSS FSSFTSSTAF
     451  ASQISIQDAP AIGDQQCLIG ESGSSLRVNS CEFPQYTTMS PVGEYPFEST
     501  EASLSSTLTP VGNVPQRIKA HLVEGKSNSK DDLRIEADAS LVFEASDAQR
     551  TRRRMSLYKL *

external links for Tos3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Tos3p
1) Nath N, et al.  (2003) Yeast Pak1 kinase associates with and activates Snf1. Mol Cell Biol 23(11):3909-17
2) Sutherland CM, et al.  (2003) Elm1p is one of three upstream kinases for the Saccharomyces cerevisiae SNF1 complex. Curr Biol 13(15):1299-305
3) Hong SP, et al.  (2003) Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases. Proc Natl Acad Sci U S A 100(15):8839-43
4) Hedbacker K, et al.  (2004) Pak1 protein kinase regulates activation and nuclear localization of Snf1-Gal83 protein kinase. Mol Cell Biol 24(18):8255-63
5) Elbing K, et al.  (2006) Purification and characterization of the three Snf1-activating kinases of Saccharomyces cerevisiae. Biochem J 393(Pt 3):797-805
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61