XRN1/YGL173C Protein Information Help

Standard Name Xrn1p 1
Systematic Name Ygl173cp
Alias Dst2p , Rar5p , Sep1p , Ski1p 2 , Kem1p 3
ORF Classification Verified
Description Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved in mRNA decay; plays a role in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance; activated by the scavenger decapping enzyme Dcs1p (1, 4, 5, 6, 7, 8, 9)
Name Description eXoRiboNuclease 1
Experimental Data
Molecules/cell 11700 10
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,528
Molecular Weight (Da) 175,458
Isoelectric Point (pI) 7.5

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Xrn1p (InterPro)
Physical Interactions There are 153 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012DD97 | P22147
MIPS: YGL173C
NCBI: 125342 | 1322778 | 172580 | 172775 | 173192 | 3847 | 4413 | 6321265 | 695728 | NP_011342.1
GenBank/EMBL/DDBJ: DAA07940.1 | M36725 | M58367 | M90097 | X54717 | X61181 | X84705 | Z72695
External Classifications EC: 3.1.11.- [Exodeoxyribonucleases Producing 5'-Phosphomonoesters]
Amino Acid Sequence (or in FASTA format)
 
       1  MGIPKFFRYI SERWPMILQL IEGTQIPEFD NLYLDMNSIL HNCTHGNDDD
      51  VTKRLTEEEV FAKICTYIDH LFQTIKPKKI FYMAIDGVAP RAKMNQQRAR
     101  RFRTAMDAEK ALKKAIENGD EIPKGEPFDS NSITPGTEFM AKLTKNLQYF
     151  IHDKISNDSK WREVQIIFSG HEVPGEGEHK IMNFIRHLKS QKDFNQNTRH
     201  CIYGLDADLI MLGLSTHGPH FALLREEVTF GRRNSEKKSL EHQNFYLLHL
     251  SLLREYMELE FKEIADEMQF EYNFERILDD FILVMFVIGN DFLPNLPDLH
     301  LNKGAFPVLL QTFKEALLHT DGYINEHGKI NLKRLGVWLN YLSQFELLNF
     351  EKDDIDVEWF NKQLENISLE GERKRQRVGK KLLVKQQKKL IGSIKPWLME
     401  QLQEKLSPDL PDEEIPTLEL PKDLDMKDHL EFLKEFAFDL GLFITHSKSK
     451  GSYSLKMDLD SINPDETEEE FQNRVNSIRK TIKKYQNAII VEDKEELETE
     501  KTIYNERFER WKHEYYHDKL KFTTDSEEKV RDLAKDYVEG LQWVLYYYYR
     551  GCPSWSWYYP HHYAPRISDL AKGLDQDIEF DLSKPFTPFQ QLMAVLPERS
     601  KNLIPPAFRP LMYDEQSPIH DFYPAEVQLD KNGKTADWEA VVLISFVDEK
     651  RLIEAMQPYL RKLSPEEKTR NQFGKDLIYS FNPQVDNLYK SPLGGIFSDI
     701  EHNHCVEKEY ITIPLDSSEI RYGLLPNAKL GAEMLAGFPT LLSLPFTSSL
     751  EYNETMVFQQ PSKQQSMVLQ ITDIYKTNNV TLEDFSKRHL NKVIYTRWPY
     801  LRESKLVSLT DGKTIYEYQE SNDKKKFGFI TKPAETQDKK LFNSLKNSML
     851  RMYAKQKAVK IGPMEAIATV FPVTGLVRDS DGGYIKTFSP TPDYYPLQLV
     901  VESVVNEDER YKERGPIPIE EEFPLNSKVI FLGDYAYGGE TTIDGYSSDR
     951  RLKITVEKKF LDSEPTIGKE RLQMDHQAVK YYPSYIVSKN MHLHPLFLSK
    1001  ITSKFMITDA TGKHINVGIP VKFEARHQKV LGYARRNPRG WEYSNLTLNL
    1051  LKEYRQTFPD FFFRLSKVGN DIPVLEDLFP DTSTKDAMNL LDGIKQWLKY
    1101  VSSKFIAVSL ESDSLTKTSI AAVEDHIMKY AANIEGHERK QLAKVPREAV
    1151  LNPRSSFALL RSQKFDLGDR VVYIQDSGKV PIFSKGTVVG YTTLSSSLSI
    1201  QVLFDHEIVA GNNFGGRLRT NRGLGLDASF LLNITNRQFI YHSKASKKAL
    1251  EKKKQSNNRN NNTKTAHKTP SKQQSEEKLR KERAHDLLNF IKKDTNEKNS
    1301  ESVDNKSMGS QKDSKPAKKV LLKRPAQKSS ENVQVDLANF EKAPLDNPTV
    1351  AGSIFNAVAN QYSDGIGSNL NIPTPPHPMN VVGGPIPGAN DVADVGLPYN
    1401  IPPGFMTHPN GLHPLHPHQM PYPNMNGMSI PPPAPHGFGQ PISFPPPPPM
    1451  TNVSDQGSRI VVNEKESQDL KKFINGKQHS NGSTIGGETK NSRKGEIKPS
    1501  SGTNSTECQS PKSQSNAADR DNKKDEST*

external links for Xrn1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Xrn1p
1) Larimer FW and Stevens A  (1990) Disruption of the gene XRN1, coding for a 5'----3' exoribonuclease, restricts yeast cell growth. Gene 95(1):85-90
2) Toh-E A, et al.  (1978) Chromosomal superkiller mutants of Saccharomyces cerevisiae. J Bacteriol 136(3):1002-7
3) Kim J, et al.  (1990) kem mutations affect nuclear fusion in Saccharomyces cerevisiae. Genetics 126(4):799-812
4) Solinger JA, et al.  (1999) Active-site mutations in the Xrn1p exoribonuclease of Saccharomyces cerevisiae reveal a specific role in meiosis. Mol Cell Biol 19(9):5930-42
5) Geerlings TH, et al.  (2000) The final step in the formation of 25S rRNA in Saccharomyces cerevisiae is performed by 5'-->3' exonucleases. RNA 6(12):1698-703
6) Kim J and Kim J  (2002) KEM1 is involved in filamentous growth of Saccharomyces cerevisiae. FEMS Microbiol Lett 216(1):33-8
7) Sheth U and Parker R  (2003) Decapping and decay of messenger RNA occur in cytoplasmic processing bodies. Science 300(5620):805-8
8) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
9) Sinturel F, et al.  (2012) Activation of 5'-3' exoribonuclease Xrn1 by cofactor Dcs1 is essential for mitochondrial function in yeast. Proc Natl Acad Sci U S A 109(21):8264-9
10) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41