NUP49/YGL172W Protein Information Help

Standard Name Nup49p 1
Systematic Name Ygl172wp
Alias Nsp49p
ORF Classification Verified
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) (10, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description NUclear Pore 11
Experimental Data
Molecules/cell 4760 12
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 472
Molecular Weight (Da) 49,142
Isoelectric Point (pI) 6.06

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Nup49p (InterPro)
Physical Interactions There are 107 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013071E | Q02199
MIPS: YGL172W
NCBI: 1322776 | 400618 | 4056 | 4078 | 6321266 | 677857 | NP_011343.1 | NM_001181037.1
GenBank/EMBL/DDBJ: CAA48229.1 | CAA59181.1 | CAA78758.1 | CAA96884.1 | DAA07941.1 | X68109 | X84705 | Z15040 | Z72694
Amino Acid Sequence (or in FASTA format)
       1  MFGLNKASST PAGGLFGQAS GASTGNANTG FSFGGTQTGQ NTGPSTGGLF
      51  GAKPAGSTGG LGASFGQQQQ QSQTNAFGGS ATTGGGLFGN KPNNTANTGG
     101  GLFGANSNSN SGSLFGSNNA QTSRGLFGNN NTNNINNSSS GMNNASAGLF
     151  GSKPAGGTSL FGNTSTSSAP AQNQGMFGAK PAGTSLFGNN AGNTTTGGGL
     201  FGSKPTGATS LFGSSNNNNN NNNSNNIMSA SGGLFGNQQQ QLQQQPQMQC
     251  ALQNLSQLPI TPMTRISELP PQIRQEIEQL DQYIQKQVQI SHHLKADTID
     301  HDELIDSIPR DVAYLLKSES ATSQYLKQDL KKISSFKSLI DEDLLDTQTF
     351  SVLLQQLLTP GSKISSNDLD KFFQKKIHLY EKKLEDYCRI LSDIETAVNG
     401  IDTDLFGAPN NPNSTAITAD LGSSEAENLL QLKTGLAAIV STVIEEFTLF
     451  MDIAERIAVL HQKTKTLASL SI*                             

external links for Nup49p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
Candida (CGD) CYC2008 (complexes) MIPS YPL+
YGOB Complexome Pfam domains YeastGFP
YOGY DIP YeastRC Structure Prediction (Seattle) YeastRC Public Image Repository

GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Nup49p
1) Wente SR, et al.  (1992) A new family of yeast nuclear pore complex proteins. J Cell Biol 119(4):705-23
2) Grandi P, et al.  (1993) Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J 12(8):3061-71
3) Hurt E, et al.  (1999) A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants. J Cell Biol 144(3):389-401
4) Sharma K, et al.  (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol Cell Biol 16(1):294-301
5) Doye V, et al.  (1994) A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution. EMBO J 13(24):6062-75
6) Stage-Zimmermann T, et al.  (2000) Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Mol Biol Cell 11(11):3777-89
7) Allen NP, et al.  (2001) Proteomic analysis of nucleoporin interacting proteins. J Biol Chem 276(31):29268-74
8) Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
9) Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
10) Frey S and Gorlich D  (2009) FG/FxFG as well as GLFG repeats form a selective permeability barrier with self-healing properties. EMBO J 28(17):2554-67
11) Davis LI and Fink GR  (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
12) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41