ROK1/YGL171W Protein Information

Standard Name	Rok1p 1
Systematic Name	Ygl171wp
ORF Classification	Verified
Description	RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation (2, 3, 4)
Name Description	Rescuer Of Kem1 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	564
Molecular Weight (Da)	63,652
Isoelectric Point (pI)	9.86

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rok1p (InterPro)
Physical Interactions	There are 29 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053027 \| P45818 MIPS: YGL171W NCBI: 1173121 \| 1322774 \| 607182 \| 6321267 \| 971382 \| NP_011344.1 GenBank/EMBL/DDBJ: DAA07942.1 \| X85757 \| Z34901 \| Z72693
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.13 [RNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MDIFRVLTRG ASVKKESGPK AKAADYSVIN GNDENHKEDN NESQIVKELD 51 FFRNKRIISK VEDDREKTTE NDSPNKEEKS GNDDGLIKPV ITNTVEASAL 101 RKSYKGNVSG IDIPLPIGSF EDLISRFSFD KRLLNNLIEN GFTEPTPIQC 151 ECIPVALNNR DVLACGPTGS GKTLAFLIPL VQQIIDDKQT AGLKGLIISP 201 TKELANQIFI ECFKLSYKIF LEKKRPLQVA LLSKSLGAKL KNKVVSDKKY 251 DIIISTPLRL IDVVKNEALD LSKVKHLIFD EADKLFDKTF VEQSDDILSA 301 CREPSLRKAM FSATIPSNVE EIAQSIMMDP VRVIIGHKEA ANTNIEQKLI 351 FCGNEEGKLI AIRQLVQEGE FKPPIIIFLE SITRAKALYH ELMYDRINVD 401 VIHAERTALQ RDRIIERFKT GELWCLICTD VLARGIDFKG VNLVINYDVP 451 GSSQAYVHRI GRTGRGGRSG KAITFYTKQD SVAIKPIINV MKQSGCEVSE 501 WMDKMAKMTR KEKESIKNGK AHKERKQITT VPKMDKAKRR RQQEMIAASK 551 RRKNEELSKK HFSK*

Amino Acid Sequence (or in FASTA format)

       1  MDIFRVLTRG ASVKKESGPK AKAADYSVIN GNDENHKEDN NESQIVKELD
      51  FFRNKRIISK VEDDREKTTE NDSPNKEEKS GNDDGLIKPV ITNTVEASAL
     101  RKSYKGNVSG IDIPLPIGSF EDLISRFSFD KRLLNNLIEN GFTEPTPIQC
     151  ECIPVALNNR DVLACGPTGS GKTLAFLIPL VQQIIDDKQT AGLKGLIISP
     201  TKELANQIFI ECFKLSYKIF LEKKRPLQVA LLSKSLGAKL KNKVVSDKKY
     251  DIIISTPLRL IDVVKNEALD LSKVKHLIFD EADKLFDKTF VEQSDDILSA
     301  CREPSLRKAM FSATIPSNVE EIAQSIMMDP VRVIIGHKEA ANTNIEQKLI
     351  FCGNEEGKLI AIRQLVQEGE FKPPIIIFLE SITRAKALYH ELMYDRINVD
     401  VIHAERTALQ RDRIIERFKT GELWCLICTD VLARGIDFKG VNLVINYDVP
     451  GSSQAYVHRI GRTGRGGRSG KAITFYTKQD SVAIKPIINV MKQSGCEVSE
     501  WMDKMAKMTR KEKESIKNGK AHKERKQITT VPKMDKAKRR RQQEMIAASK
     551  RRKNEELSKK HFSK*

external links for Rok1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rok1p

1)	Song Y, et al. (1995) ROK1, a high-copy-number plasmid suppressor of kem1, encodes a putative ATP-dependent RNA helicase in Saccharomyces cerevisiae. Gene 166(1):151-4
2)	Venema J, et al. (1997) Rok1p is a putative RNA helicase required for rRNA processing. Mol Cell Biol 17(6):3398-407
3)	Garcia I and Uhlenbeck OC (2008) Differential RNA-Dependent ATPase Activities of Four rRNA Processing Yeast DEAD-Box Proteins (dagger). Biochemistry 47(47):12562-73
4)	Jeon S and Kim J (2010) Upstream open reading frames regulate the cell cycle-dependent expression of the RNA helicase Rok1 in Saccharomyces cerevisiae. FEBS Lett 584(22):4593-8