CUP2/YGL166W Protein Information

Standard Name	Cup2p 1
Systematic Name	Ygl166wp
Alias	Ace1p 2, 3
ORF Classification	Verified
Description	Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication (3, 4, 5)

Experimental Data
Molecules/cell	1760 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	225
Molecular Weight (Da)	24,425
Isoelectric Point (pI)	7.69

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cup2p (InterPro)
Physical Interactions	There are 13 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001251F3 \| P15315 MIPS: YGL166W NCBI: 113021 \| 1322764 \| 170974 \| 170978 \| 171340 \| 398364351 \| 45269531 \| 728691 \| 971389 \| NP_011349.3 GenBank/EMBL/DDBJ: DAA07947.1 \| AY557820 \| M22580 \| M24390 \| M28520 \| X85757 \| Z48618 \| Z72688

Amino Acid Sequence (or in FASTA format)
1 MVVINGVKYA CETCIRGHRA AQCTHTDGPL QMIRRKGRPS TTCGHCKELR 51 RTKNFNPSGG CMCASARRPA VGSKEDETRC RCDEGEPCKC HTKRKSSRKS 101 KGGSCHRRAN DEAAHVNGLG IADLDVLLGL NGRSSDVDMT TTLPSLKPPL 151 QNGEIKADSI DNLDLASLDP LEQSPSISME PVSINETGSA YTTTNTALND 201 IDIPFSINEL NELYKQVSSH NSHSQ*

Amino Acid Sequence (or in FASTA format)

       1  MVVINGVKYA CETCIRGHRA AQCTHTDGPL QMIRRKGRPS TTCGHCKELR
      51  RTKNFNPSGG CMCASARRPA VGSKEDETRC RCDEGEPCKC HTKRKSSRKS
     101  KGGSCHRRAN DEAAHVNGLG IADLDVLLGL NGRSSDVDMT TTLPSLKPPL
     151  QNGEIKADSI DNLDLASLDP LEQSPSISME PVSINETGSA YTTTNTALND
     201  IDIPFSINEL NELYKQVSSH NSHSQ*

external links for Cup2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cup2p

1)	Welch, J.W. and Fogel, S. (1985) Personal Communication, Mortimer Map Edition 9
2)	Thiele DJ (1988) ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene. Mol Cell Biol 8(7):2745-52
3)	Buchman C, et al. (1989) The CUP2 gene product, regulator of yeast metallothionein expression, is a copper-activated DNA-binding protein. Mol Cell Biol 9(9):4091-5
4)	Szczypka MS and Thiele DJ (1989) A cysteine-rich nuclear protein activates yeast metallothionein gene transcription. Mol Cell Biol 9(2):421-9
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41