RAD54/YGL163C Protein Information Help

Standard Name Rad54p 1, 2
Systematic Name Ygl163cp
Alias Xrs1p
ORF Classification Verified
Description DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress (3, 4, 5, 6)
Name Description RADiation sensitive
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 898
Molecular Weight (Da) 101,753
Isoelectric Point (pI) 9.63

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rad54p (InterPro)
Physical Interactions There are 31 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000133038 | P32863
MIPS: YGL163C
NCBI: 1322760 | 172343 | 417577 | 6321275 | 728692 | NP_011352.1 | NM_001181028.1
GenBank/EMBL/DDBJ: DAA07949.1 | M63232 | Z48618 | Z72685
External Classifications EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]
EC: 3.6.4.-
Amino Acid Sequence (or in FASTA format)
 
       1  MARRRLPDRP PNGIGAGERP RLVPRPINVQ DSVNRLTKPF RVPYKNTHIP
      51  PAAGRIATGS DNIVGGRSLR KRSATVCYSG LDINADEAEY NSQDISFSQL
     101  TKRRKDALSA QRLAKDPTRL SHIQYTLRRS FTVPIKGYVQ RHSLPLTLGM
     151  KKKITPEPRP LHDPTDEFAI VLYDPSVDGE MIVHDTSMDN KEEESKKMIK
     201  STQEKDNINK EKNSQEERPT QRIGRHPALM TNGVRNKPLR ELLGDSENSA
     251  ENKKKFASVP VVIDPKLAKI LRPHQVEGVR FLYRCVTGLV MKDYLEAEAF
     301  NTSSEDPLKS DEKALTESQK TEQNNRGAYG CIMADEMGLG KTLQCIALMW
     351  TLLRQGPQGK RLIDKCIIVC PSSLVNNWAN ELIKWLGPNT LTPLAVDGKK
     401  SSMGGGNTTV SQAIHAWAQA QGRNIVKPVL IISYETLRRN VDQLKNCNVG
     451  LMLADEGHRL KNGDSLTFTA LDSISCPRRV ILSGTPIQND LSEYFALLSF
     501  SNPGLLGSRA EFRKNFENPI LRGRDADATD KEITKGEAQL QKLSTIVSKF
     551  IIRRTNDILA KYLPCKYEHV IFVNLKPLQN ELYNKLIKSR EVKKVVKGVG
     601  GSQPLRAIGI LKKLCNHPNL LNFEDEFDDE DDLELPDDYN MPGSKARDVQ
     651  TKYSAKFSIL ERFLHKIKTE SDDKIVLISN YTQTLDLIEK MCRYKHYSAV
     701  RLDGTMSINK RQKLVDRFND PEGQEFIFLL SSKAGGCGIN LIGANRLILM
     751  DPDWNPAADQ QALARVWRDG QKKDCFIYRF ISTGTIEEKI FQRQSMKMSL
     801  SSCVVDAKED VERLFSSDNL RQLFQKNENT ICETHETYHC KRCNAQGKQL
     851  KRAPAMLYGD ATTWNHLNHD ALEKTNDHLL KNEHHYNDIS FAFQYISH*

external links for Rad54p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Rad54p
1) Dowling, E.L.  (1985) Ph.D. thesis
2) Game, J.  (1985) Personal Communication, Mortimer Map Edition 9
3) Petukhova G, et al.  (1999) Yeast Rad54 promotes Rad51-dependent homologous DNA pairing via ATP hydrolysis-driven change in DNA double helix conformation. J Biol Chem 274(41):29453-62
4) Clever B, et al.  (1997) Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins. EMBO J 16(9):2535-44
5) Symington LS  (2002) Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev 66(4):630-70, table of contents
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76