AMS1/YGL156W Protein Information Help

Standard Name Ams1p 1
Systematic Name Ygl156wp
ORF Classification Verified
Description Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway (2, 3, 4)
Name Description Alpha-MannoSidase 1
Experimental Data
Molecules/cell 319 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,083
Molecular Weight (Da) 124,498
Isoelectric Point (pI) 7.22

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ams1p (InterPro)
Physical Interactions There are 16 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000531EA | P22855
MIPS: YGL156W
NCBI: 1322746 | 1346479 | 171058 | 6321282 | 728694 | NP_011359.1
GenBank/EMBL/DDBJ: DAA07956.1 | M29146 | Z48618 | Z72678
External Classifications EC: 3.2.1.24 [Alpha-mannosidase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSSEDIIYDP QFKPVQGIYE NRLRQFIDTG GDYHDLNLPK FYDKKRISLD
      51  HDHVKVWWYQ VSFERGSSPV SPDKRPSWKS IIERDKKGEL EFREANINQP
     101  FGPSWSTTWF KVKISLPEDW VKSNEQLLFQ WDCSNEGIVI DPKTLIPVTA
     151  FSGGERTEYV LPKTSDGKHF FYIEAGNNGM FGCGAGSTIN PPDDNRFFHL
     201  RKADIVWPDL DARALYIDFW MLGDAARELP GDSWQKHQAR QLGNAVMNLF
     251  DPNDRSSVRK CRELLQREYF DSFLESSKVY EQGESQVLTN VYGIGNCHID
     301  TAWLWPFAET RRKIVRSWSS QCTLMDRFPE YKFVASQAQQ FKWLLEDHPE
     351  FFNKVLIPKI QQSQFFAVGG TWVENDTNIP SGESLARQFF FGQRFFLKHF
     401  GLKSKIFWLP DTFGYSSQMP QLCRLSGIDK FLTQKLSWNN INSFPHSTFN
     451  WAGIDGSQLL THMPPGNTYT ADSHFGDVLR TAKQNKTPEY YGSGLMLYGK
     501  GDGGGGPTEE MLQKMRRIRS MNNRNGNVIP KLQVGITVDE FYDDILKRTN
     551  QGHDLPTWSG ELYFEFHRGT YTSQAQTKKL MRLSEIKLHD LEWIAAKTSV
     601  LYPDSYKYPS KQINELWENV LLCQFHDVLP GSCIEMVYKY EAVPMLHNVV
     651  KECTSLIDKT VQFLQSQSKA DLVEMRTLTW SKPEKVSEEC SLNGSYTSSV
     701  TGYDDYIVLA NGKLKVIICK KTGVITSITD ETLGVEYLDT EHGRNKLGAN
     751  QFVIYDDKPL GWQAWDTELY SVNQYKYVTK PKKVQVSCNT KEKCAVEVIF
     801  QISEKCKIKS VISLNATAVT DAKLSKVDIS TTVENWDARN KFLKVEFPVN
     851  IRNDFASYET QFGITKRPTH YNTSWDVAKF EVCHHKFADY SEYSKGVSIL
     901  NDCKYGFSTH GNLMRLSLLR SPKAPDAHAD MGTHEIKYAI YPHRGALSSD
     951  TVKLAHEFNY CFKYKLPKDI GMNFDDIISI SGDENVILSN IKRGEDDSAV
    1001  KSNYSLNPRD EQSIVVRVYE SLGGESFASL NTTLNLKRIE KVDNLEMKVY
    1051  KSLTATRDES NHAINRIPIK LRPFEIASFR LYF*

external links for Ams1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Ams1p
1) Yoshihisa T and Anraku Y  (1989) Nucleotide sequence of AMS1, the structure gene of vacuolar alpha-mannosidase of Saccharomyces cerevisiae. Biochem Biophys Res Commun 163(2):908-15
2) Yoshihisa T and Anraku Y  (1990) A novel pathway of import of alpha-mannosidase, a marker enzyme of vacuolar membrane, in Saccharomyces cerevisiae. J Biol Chem 265(36):22418-25
3) Hutchins MU and Klionsky DJ  (2001) Vacuolar localization of oligomeric alpha-mannosidase requires the cytoplasm to vacuole targeting and autophagy pathway components in Saccharomyces cerevisiae. J Biol Chem 276(23):20491-8
4) Chantret I, et al.  (2003) Free-oligosaccharide control in the yeast Saccharomyces cerevisiae: roles for peptide:N-glycanase (Png1p) and vacuolar mannosidase (Ams1p). Biochem J 373(Pt 3):901-8
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41