SNF4/YGL115W Protein Information Help

Standard Name Snf4p 1, 2
Systematic Name Ygl115wp
Alias Cat3p 3 , Sci1p 4
ORF Classification Verified
Description Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress (2, 5, 6, 7)
Name Description Sucrose NonFermenting 8
Experimental Data
Molecules/cell 11700 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 322
Molecular Weight (Da) 36,401
Isoelectric Point (pI) 5.52

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Snf4p (InterPro)
Physical Interactions There are 123 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052F23 | P12904
MIPS: YGL115W
NCBI: 115689 | 122921176 | 122921178 | 122921179 | 158430320 | 158430323 | 2160324 | 356624535 | 356624591 | 356624594 | 6321323 | NP_011400.1
GenBank/EMBL/DDBJ: DAA07993.1 | D16506 | M21760 | M30470 | Z72637
Amino Acid Sequence (or in FASTA format)
 
       1  MKPTQDSQEK VSIEQQLAVE SIRKFLNSKT SYDVLPVSYR LIVLDTSLLV
      51  KKSLNVLLQN SIVSAPLWDS KTSRFAGLLT TTDFINVIQY YFSNPDKFEL
     101  VDKLQLDGLK DIERALGVDQ LDTASIHPSR PLFEACLKML ESRSGRIPLI
     151  DQDEETHREI VVSVLTQYRI LKFVALNCRE THFLKIPIGD LNIITQDNMK
     201  SCQMTTPVID VIQMLTQGRV SSVPIIDENG YLINVYEAYD VLGLIKGGIY
     251  NDLSLSVGEA LMRRSDDFEG VYTCTKNDKL STIMDNIRKA RVHRFFVVDD
     301  VGRLVGVLTL SDILKYILLG SN*

external links for Snf4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Snf4p
1) Neigeborn L and Carlson M  (1984) Genes affecting the regulation of SUC2 gene expression by glucose repression in Saccharomyces cerevisiae. Genetics 108(4):845-58
2) Celenza JL, et al.  (1989) Molecular analysis of the SNF4 gene of Saccharomyces cerevisiae: evidence for physical association of the SNF4 protein with the SNF1 protein kinase. Mol Cell Biol 9(11):5045-54
3) Schuller HJ and Entian KD  (1988) Molecular characterization of yeast regulatory gene CAT3 necessary for glucose derepression and nuclear localization of its product. Gene 67(2):247-57
4) Blazquez MA and Gancedo C  (1994) Identification of extragenic suppressors of the cif1 mutation in Saccharomyces cerevisiae. Curr Genet 25(2):89-94
5) Simon M, et al.  (1992) Control of peroxisome proliferation in Saccharomyces cerevisiae by ADR1, SNF1 (CAT1, CCR1) and SNF4 (CAT3). Yeast 8(4):303-9
6) Celenza JL and Carlson M  (1989) Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein. Mol Cell Biol 9(11):5034-44
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Carlson M, et al.  (1981) Mutants of yeast defective in sucrose utilization. Genetics 98(1):25-40
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41