ARC1/YGL105W Protein Information

Standard Name	Arc1p
Systematic Name	Ygl105wp
ORF Classification	Verified
Description	Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description	Acyl-RNA-Complex

Experimental Data
Molecules/cell	57700 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	376
Molecular Weight (Da)	42,084
Isoelectric Point (pI)	8.01

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Arc1p (InterPro)
Physical Interactions	There are 85 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012AEF3 \| P46672 MIPS: YGL105W NCBI: 1002712 \| 114794462 \| 114794463 \| 114794464 \| 114794465 \| 114794466 \| 114794467 \| 114794468 \| 114794469 \| 114794470 \| 114794471 \| 114794472 \| 114794473 \| 114794474 \| 114794475 \| 114794476 \| 114794477 \| 114794478 \| 114794479 \| 114794480 \| 114794481 \| 114794489 \| 114794500 \| 114794502 \| 1310719 \| 1322648 \| 1620460 \| 1730165 \| 45270886 \| 6321333 \| NP_011410.1 GenBank/EMBL/DDBJ: DAA08002.1 \| AY558498 \| U31348 \| X95481 \| X97644 \| Z72627

Amino Acid Sequence (or in FASTA format)
1 MSDLVTKFES LIISKYPVSF TKEQSAQAAQ WESVLKSGQI QPHLDQLNLV 51 LRDNTFIVST LYPTSTDVHV FEVALPLIKD LVASSKDVKS TYTTYRHILR 101 WIDYMQNLLE VSSTDKLEIN HDLDLPHEVI EKKKKAPAGG AADAAAKADE 151 DVSKKAKKQD HPRGKPDEET LKKLREEAKA KKAAKKAANA KQQQEQQNKA 201 PEKPKPSAID FRVGFIQKAI KHPDADSLYV STIDVGDEEG PRTVCSGLVK 251 HFPLDAMQER YVVVVCNLKP VNMRGIKSTA MVLCGSNDDK VEFVEPPKDS 301 KAGDKVFFEG FGDEAPMKQL NPKKKIWEHL QPHFTTNDGL EVIFKDEEEK 351 DHPVRKLTNA KGESFKVASI ANAQVR*

Amino Acid Sequence (or in FASTA format)

       1  MSDLVTKFES LIISKYPVSF TKEQSAQAAQ WESVLKSGQI QPHLDQLNLV
      51  LRDNTFIVST LYPTSTDVHV FEVALPLIKD LVASSKDVKS TYTTYRHILR
     101  WIDYMQNLLE VSSTDKLEIN HDLDLPHEVI EKKKKAPAGG AADAAAKADE
     151  DVSKKAKKQD HPRGKPDEET LKKLREEAKA KKAAKKAANA KQQQEQQNKA
     201  PEKPKPSAID FRVGFIQKAI KHPDADSLYV STIDVGDEEG PRTVCSGLVK
     251  HFPLDAMQER YVVVVCNLKP VNMRGIKSTA MVLCGSNDDK VEFVEPPKDS
     301  KAGDKVFFEG FGDEAPMKQL NPKKKIWEHL QPHFTTNDGL EVIFKDEEEK
     351  DHPVRKLTNA KGESFKVASI ANAQVR*

external links for Arc1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Arc1p

1)	Frantz JD and Gilbert W (1995) A novel yeast gene product, G4p1, with a specific affinity for quadruplex nucleic acids. J Biol Chem 270(35):20692-7
2)	Simos G, et al. (1996) The yeast protein Arc1p binds to tRNA and functions as a cofactor for the methionyl- and glutamyl-tRNA synthetases. EMBO J 15(19):5437-48
3)	Simos G, et al. (1998) A conserved domain within Arc1p delivers tRNA to aminoacyl-tRNA synthetases. Mol Cell 1(2):235-42
4)	Galani K, et al. (2001) The intracellular location of two aminoacyl-tRNA synthetases depends on complex formation with Arc1p. EMBO J 20(23):6889-98
5)	Deinert K, et al. (2001) Arc1p organizes the yeast aminoacyl-tRNA synthetase complex and stabilizes its interaction with the cognate tRNAs. J Biol Chem 276(8):6000-8
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41