LSG1/YGL099W Protein Information

Standard Name	Lsg1p 1
Systematic Name	Ygl099wp
Alias	Kre35p
ORF Classification	Verified
Description	Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm (1, 2)
Name Description	Large-Subunit Gtpase 1

Experimental Data
Molecules/cell	19400 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	640
Molecular Weight (Da)	72,727
Isoelectric Point (pI)	9.45

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Lsg1p (InterPro)
Physical Interactions	There are 134 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E37 \| P53145 MIPS: YGL099W NCBI: 1322637 \| 1723894 \| 398364525 \| NP_011416.3 GenBank/EMBL/DDBJ: DAA08007.1 \| Z72621

Amino Acid Sequence (or in FASTA format)
1 MPPKEAPKKW KAPKGPKPTH RKNKNKLELG RAIKYARQKE NAIEYLPDGE 51 MRFTTDKHEA NWVKLRSVTQ ESALDEFLST AALADKDFTA DRHSNVKIIR 101 MDSGNDSATS QGFSMTNEQR GNLNAKQRAL AKDLIVPRRP EWNEGMSKFQ 151 LDRQEKEAFL EWRRKLAHLQ ESNEDLLLTP FERNIEVWKQ LWRVVERSDL 201 VVQIVDARNP LLFRSVDLER YVKESDDRKA NLLLVNKADL LTKKQRIAWA 251 KYFISKNISF TFYSALRANQ LLEKQKEMGE DYREQDFEEA DKEGFDADEK 301 VMEKVKILSI DQLEELFLSK APNEPLLPPL PGQPPLINIG LVGYPNVGKS 351 STINSLVGAK KVSVSSTPGK TKHFQTIKLS DSVMLCDCPG LVFPNFAYNK 401 GELVCNGVLP IDQLRDYIGP AGLVAERIPK YYIEAIYGIH IQTKSRDEGG 451 NGDIPTAQEL LVAYARARGY MTQGYGSADE PRASRYILKD YVNGKLLYVN 501 PPPHLEDDTP YTREECEEFN KDLYVFDRLP DTRKEQVQNA AKAKGIDIVD 551 LARDLNQLTF SAHTGGDTQK EAKSVTHGGK QAALYNAAED LDRDFFKMNN 601 VEGRLSTPFH KVQNSSAGKR HNKKNKSKNA KSKVFSIENN *

Amino Acid Sequence (or in FASTA format)

       1  MPPKEAPKKW KAPKGPKPTH RKNKNKLELG RAIKYARQKE NAIEYLPDGE
      51  MRFTTDKHEA NWVKLRSVTQ ESALDEFLST AALADKDFTA DRHSNVKIIR
     101  MDSGNDSATS QGFSMTNEQR GNLNAKQRAL AKDLIVPRRP EWNEGMSKFQ
     151  LDRQEKEAFL EWRRKLAHLQ ESNEDLLLTP FERNIEVWKQ LWRVVERSDL
     201  VVQIVDARNP LLFRSVDLER YVKESDDRKA NLLLVNKADL LTKKQRIAWA
     251  KYFISKNISF TFYSALRANQ LLEKQKEMGE DYREQDFEEA DKEGFDADEK
     301  VMEKVKILSI DQLEELFLSK APNEPLLPPL PGQPPLINIG LVGYPNVGKS
     351  STINSLVGAK KVSVSSTPGK TKHFQTIKLS DSVMLCDCPG LVFPNFAYNK
     401  GELVCNGVLP IDQLRDYIGP AGLVAERIPK YYIEAIYGIH IQTKSRDEGG
     451  NGDIPTAQEL LVAYARARGY MTQGYGSADE PRASRYILKD YVNGKLLYVN
     501  PPPHLEDDTP YTREECEEFN KDLYVFDRLP DTRKEQVQNA AKAKGIDIVD
     551  LARDLNQLTF SAHTGGDTQK EAKSVTHGGK QAALYNAAED LDRDFFKMNN
     601  VEGRLSTPFH KVQNSSAGKR HNKKNKSKNA KSKVFSIENN *

external links for Lsg1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Lsg1p

1)	Kallstrom G, et al. (2003) The putative GTPases Nog1p and Lsg1p are required for 60S ribosomal subunit biogenesis and are localized to the nucleus and cytoplasm, respectively. Mol Cell Biol 23(12):4344-55
2)	Hedges J, et al. (2005) Release of the export adapter, Nmd3p, from the 60S ribosomal subunit requires Rpl10p and the cytoplasmic GTPase Lsg1p. EMBO J 24(3):567-79
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41