DBP3/YGL078C Protein Information

Standard Name	Dbp3p
Systematic Name	Ygl078cp
ORF Classification	Verified
Description	RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype (1, 2)
Name Description	Dead Box Protein

Experimental Data
Molecules/cell	38900 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	523
Molecular Weight (Da)	58,826
Isoelectric Point (pI)	9.91

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Dbp3p (InterPro)
Physical Interactions	There are 47 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000128F2B \| P20447 MIPS: YGL078C NCBI: 1322595 \| 1708151 \| 172582 \| 398364607 \| NP_011437.3 \| NM_001180943.3 GenBank/EMBL/DDBJ: DAA08027.1 \| M80437 \| Z72600
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.13 [RNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MTKEEIADKK RKVVDEEVIE KKKSKKHKKD KKDKKEKKDK KHKKHKKEKK 51 GEKEVEVPEK ESEKKPEPTS AVASEFYVQS EALTSLPQSD IDEYFKENEI 101 AVEDSLDLAL RPLLSFDYLS LDSSIQAEIS KFPKPTPIQA VAWPYLLSGK 151 DVVGVAETGS GKTFAFGVPA ISHLMNDQKK RGIQVLVISP TRELASQIYD 201 NLIVLTDKVG MQCCCVYGGV PKDEQRIQLK KSQVVVATPG RLLDLLQEGS 251 VDLSQVNYLV LDEADRMLEK GFEEDIKNII RETDASKRQT LMFTATWPKE 301 VRELASTFMN NPIKVSIGNT DQLTANKRIT QIVEVVDPRG KERKLLELLK 351 KYHSGPKKNE KVLIFALYKK EAARVERNLK YNGYNVAAIH GDLSQQQRTQ 401 ALNEFKSGKS NLLLATDVAA RGLDIPNVKT VINLTFPLTV EDYVHRIGRT 451 GRAGQTGTAH TLFTEQEKHL AGGLVNVLNG ANQPVPEDLI KFGTHTKKKE 501 HSAYGSFFKD VDLTKKPKKI TFD*

Amino Acid Sequence (or in FASTA format)

       1  MTKEEIADKK RKVVDEEVIE KKKSKKHKKD KKDKKEKKDK KHKKHKKEKK
      51  GEKEVEVPEK ESEKKPEPTS AVASEFYVQS EALTSLPQSD IDEYFKENEI
     101  AVEDSLDLAL RPLLSFDYLS LDSSIQAEIS KFPKPTPIQA VAWPYLLSGK
     151  DVVGVAETGS GKTFAFGVPA ISHLMNDQKK RGIQVLVISP TRELASQIYD
     201  NLIVLTDKVG MQCCCVYGGV PKDEQRIQLK KSQVVVATPG RLLDLLQEGS
     251  VDLSQVNYLV LDEADRMLEK GFEEDIKNII RETDASKRQT LMFTATWPKE
     301  VRELASTFMN NPIKVSIGNT DQLTANKRIT QIVEVVDPRG KERKLLELLK
     351  KYHSGPKKNE KVLIFALYKK EAARVERNLK YNGYNVAAIH GDLSQQQRTQ
     401  ALNEFKSGKS NLLLATDVAA RGLDIPNVKT VINLTFPLTV EDYVHRIGRT
     451  GRAGQTGTAH TLFTEQEKHL AGGLVNVLNG ANQPVPEDLI KFGTHTKKKE
     501  HSAYGSFFKD VDLTKKPKKI TFD*

external links for Dbp3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Dbp3p

1)	Weaver PL, et al. (1997) Dbp3p, a putative RNA helicase in Saccharomyces cerevisiae, is required for efficient pre-rRNA processing predominantly at site A3. Mol Cell Biol 17(3):1354-65
2)	Garcia I and Uhlenbeck OC (2008) Differential RNA-Dependent ATPase Activities of Four rRNA Processing Yeast DEAD-Box Proteins (dagger). Biochemistry 47(47):12562-73
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41