SGF73/YGL066W Protein Information Help

Standard Name Sgf73p 1
Systematic Name Ygl066wp
Alias Sca7p 2
ORF Classification Verified
Description SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay (1, 2, 3, 4, 5, 6, 7)
Name Description SaGa associated Factor, 73 kDa 1
Experimental Data
Molecules/cell 486 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 657
Molecular Weight (Da) 72,878
Isoelectric Point (pI) 9.57

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sgf73p (InterPro)
Physical Interactions There are 218 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053136 | P53165
MIPS: YGL066W
NCBI: 1322574 | 1723846 | 1945293 | 294979876 | 294979881 | 295789533 | 374073968 | 400261278 | 400261282 | 400261286 | 400261290 | 400261294 | 6321372 | NP_011449.1
GenBank/EMBL/DDBJ: DAA08036.1 | Z72587 | Z72588
Amino Acid Sequence (or in FASTA format)
 
       1  MRSGDAEIKG IKPKVIEEYS LSQGSGPSND SWKSLMSSAK DTPLQYDHMN
      51  RESLKKYFNP NAQLIEDPLD KPIQYRVCEK CGKPLALTAI VDHLENHCAG
     101  ASGKSSTDPR DESTRETIRN GVESTGRNNN DDDNSNDNNN DDDDDDDNDD
     151  NEDDDDADDD DDNSNGANYK KNDSSFNPLK RSTSMESANT PNMDTKRSKT
     201  GTPQTFSSSI KKQKKVKQRN PTEKHLIDFN KQCGVELPEG GYCARSLTCK
     251  SHSMGAKRAV SGRSKPYDVL LADYHREHQT KIGAAAEKRA KQQELQKLQK
     301  QIQKEQKKHT QQQKQGQRSK QRNVNGGKSA KNGGKSTVHN GNNINEIGHV
     351  NLTPEEETTQ VLNGVSRSFP LPLESTVLSS VRYRTKYFRM REMFASSFSV
     401  KPGYTSPGYG AIHSRVGCLD LDRTTDYKFR VRTPQPINHL TNQNLNPKQI
     451  QRLQQQRALQ AQLLSQQQQQ QQQQQQHHSP QAQAQASTQQ PTQGMVPNHF
     501  PGGATNSSFN ANVSSKQIQQ QQQQQQHKSQ DTGLTPLEIQ SQQQKLRQQQ
     551  LQQQKFEAAA SYLANATKLM QESNQDSHLS GTHNNNSSKN GNNNLMTMKA
     601  SISSPNTSVN SIQSPPSVNS VNGSGQGVST GINVSGNNGR IEVGIGNSVN
     651  PYNGRIN*

external links for Sgf73p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Sgf73p
1) Sanders SL, et al.  (2002) Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry. Mol Cell Biol 22(13):4723-38
2) McMahon SJ, et al.  (2005) Polyglutamine-expanded spinocerebellar ataxia-7 protein disrupts normal SAGA and SLIK histone acetyltransferase activity. Proc Natl Acad Sci U S A 102(24):8478-82
3) Shukla A, et al.  (2006) SAGA-associated Sgf73p facilitates formation of the preinitiation complex assembly at the promoters either in a HAT-dependent or independent manner in vivo. Nucleic Acids Res 34(21):6225-32
4) Helmlinger D, et al.  (2004) Ataxin-7 is a subunit of GCN5 histone acetyltransferase-containing complexes. Hum Mol Genet 13(12):1257-65
5) Jordan PW, et al.  (2007) Novel roles for selected genes in meiotic DNA processing. PLoS Genet 3(12):e222
6) Lee KK, et al.  (2009) Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes. Epigenetics Chromatin 2(1):2
7) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41