RAD6/YGL058W Protein Information

Standard Name	Rad6p
Systematic Name	Ygl058wp
Alias	Ubc2p 1 , Pso8p 2
ORF Classification	Verified
Description	Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p (1, 3, 4, 5)
Name Description	RADiation sensitive

Experimental Data
Molecules/cell	2770 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	172
Molecular Weight (Da)	19,705
Isoelectric Point (pI)	4.02

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rad6p (InterPro)
Physical Interactions	There are 69 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000137959 \| P06104 MIPS: YGL058W NCBI: 1322558 \| 136641 \| 172349 \| 3659954 \| 3659955 \| 3659956 \| 6321380 \| NP_011457.1 \| NM_001180923.1 GenBank/EMBL/DDBJ: DAA08044.1 \| K02962 \| Z72580
External Classifications	EC: 6.3.2.19 [Ubiquitin--protein ligase]

Amino Acid Sequence (or in FASTA format)
1 MSTPARRRLM RDFKRMKEDA PPGVSASPLP DNVMVWNAMI IGPADTPYED 51 GTFRLLLEFD EEYPNKPPHV KFLSEMFHPN VYANGEICLD ILQNRWTPTY 101 DVASILTSIQ SLFNDPNPAS PANVEAATLF KDHKSQYVKR VKETVEKSWE 151 DDMDDMDDDD DDDDDDDDDE AD*

Amino Acid Sequence (or in FASTA format)

       1  MSTPARRRLM RDFKRMKEDA PPGVSASPLP DNVMVWNAMI IGPADTPYED
      51  GTFRLLLEFD EEYPNKPPHV KFLSEMFHPN VYANGEICLD ILQNRWTPTY
     101  DVASILTSIQ SLFNDPNPAS PANVEAATLF KDHKSQYVKR VKETVEKSWE
     151  DDMDDMDDDD DDDDDDDDDE AD*

external links for Rad6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rad6p

1)	Jentsch S, et al. (1987) The yeast DNA repair gene RAD6 encodes a ubiquitin-conjugating enzyme. Nature 329(6135):131-4
2)	Rolla H, et al. (2002) Mutant pso8-1 of Saccharomyces cerevisiae, sensitive to photoactivated psoralens, UV radiation, and chemical mutagens, contains a rad6 missense mutant allele. Curr Genet 41(4):217-23
3)	Varshavsky A (1996) The N-end rule: functions, mysteries, uses. Proc Natl Acad Sci U S A 93(22):12142-9
4)	Game JC and Chernikova SB (2009) The role of RAD6 in recombinational repair, checkpoints and meiosis via histone modification. DNA Repair (Amst) 8(4):470-82
5)	Ulrich HD (2009) Regulating post-translational modifications of the eukaryotic replication clamp PCNA. DNA Repair (Amst) 8(4):461-9
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41