RPT6/YGL048C Protein Information Help

Standard Name Rpt6p 1
Systematic Name Ygl048cp
Alias Cim3p 2 , Crl3p , Scb68p , Sug1p
ORF Classification Verified
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress (3, 4, 5, 6, 7)
Name Description Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase 1
Experimental Data
Molecules/cell 4700 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 405
Molecular Weight (Da) 45,271
Isoelectric Point (pI) 9.71

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rpt6p (InterPro)
Physical Interactions There are 235 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052F99 | Q01939
MIPS: YGL048C
NCBI: 1322539 | 1709801 | 172878 | 403071970 | 4591 | 51013721 | 6321390 | NP_011467.1
GenBank/EMBL/DDBJ: DAA08053.1 | AY693135 | L01626 | X66400 | Z72570
Amino Acid Sequence (or in FASTA format)
 
       1  MTAAVTSSNI VLETHESGIK PYFEQKIQET ELKIRSKTEN VRRLEAQRNA
      51  LNDKVRFIKD ELRLLQEPGS YVGEVIKIVS DKKVLVKVQP EGKYIVDVAK
     101  DINVKDLKAS QRVCLRSDSY MLHKVLENKA DPLVSLMMVE KVPDSTYDMV
     151  GGLTKQIKEI KEVIELPVKH PELFESLGIA QPKGVILYGP PGTGKTLLAR
     201  AVAHHTDCKF IRVSGAELVQ KYIGEGSRMV RELFVMAREH APSIIFMDEI
     251  DSIGSTRVEG SGGGDSEVQR TMLELLNQLD GFETSKNIKI IMATNRLDIL
     301  DPALLRPGRI DRKIEFPPPS VAARAEILRI HSRKMNLTRG INLRKVAEKM
     351  NGCSGADVKG VCTEAGMYAL RERRIHVTQE DFELAVGKVM NKNQETAISV
     401  AKLFK*

external links for Rpt6p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Rpt6p
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Ghislain M, et al.  (1993) S. cerevisiae 26S protease mutants arrest cell division in G2/metaphase. Nature 366(6453):358-62
3) Russell SJ, et al.  (1999) Subcellular localization, stoichiometry, and protein levels of 26 S proteasome subunits in yeast. J Biol Chem 274(31):21943-52
4) Xie Y and Varshavsky A  (2000) Physical association of ubiquitin ligases and the 26S proteasome. Proc Natl Acad Sci U S A 97(6):2497-502
5) Glickman MH, et al.  (1999) Functional analysis of the proteasome regulatory particle. Mol Biol Rep 26(1-2):21-8
6) Rubin DM, et al.  (1998) Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO J 17(17):4909-19
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41