RNA15/YGL044C Protein Information

Standard Name	Rna15p
Systematic Name	Ygl044cp
ORF Classification	Verified
Description	Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping (1, 2, 3, 4, 5)
Name Description	RNA synthesis

Experimental Data
Molecules/cell	6350 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	296
Molecular Weight (Da)	32,790
Isoelectric Point (pI)	4.75

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rna15p (InterPro)
Physical Interactions	There are 82 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052DDF \| P25299 MIPS: YGL044C NCBI: 1322531 \| 133144 \| 172434 \| 285803163 \| 285803165 \| 285803166 \| 301598361 \| 330689288 \| 6321394 \| NP_011471.1 GenBank/EMBL/DDBJ: DAA08056.1 \| M73462 \| Z72566

Amino Acid Sequence (or in FASTA format)
1 MNRQSGVNAG VQNNPPSRVV YLGSIPYDQT EEQILDLCSN VGPVINLKMM 51 FDPQTGRSKG YAFIEFRDLE SSASAVRNLN GYQLGSRFLK CGYSSNSDIS 101 GVSQQQQQQY NNINGNNNNN GNNNNNSNGP DFQNSGNANF LSQKFPELPS 151 GIDVNINMTT PAMMISSELA KKPKEVQLKF LQKFQEWTRA HPEDAVSLLE 201 LCPQLSFVTA ELLLTNGICK VDDLIPLASR PQEEASATNN NSVNEVVDPA 251 VLNKQKELLK QVLQLNDSQI SILPDDERMA IWDLKQKALR GEFGAF*

Amino Acid Sequence (or in FASTA format)

       1  MNRQSGVNAG VQNNPPSRVV YLGSIPYDQT EEQILDLCSN VGPVINLKMM
      51  FDPQTGRSKG YAFIEFRDLE SSASAVRNLN GYQLGSRFLK CGYSSNSDIS
     101  GVSQQQQQQY NNINGNNNNN GNNNNNSNGP DFQNSGNANF LSQKFPELPS
     151  GIDVNINMTT PAMMISSELA KKPKEVQLKF LQKFQEWTRA HPEDAVSLLE
     201  LCPQLSFVTA ELLLTNGICK VDDLIPLASR PQEEASATNN NSVNEVVDPA
     251  VLNKQKELLK QVLQLNDSQI SILPDDERMA IWDLKQKALR GEFGAF*

external links for Rna15p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rna15p

1)	Gross S and Moore C (2001) Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I. Proc Natl Acad Sci U S A 98(11):6080-5
2)	Gross S and Moore CL (2001) Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation. Mol Cell Biol 21(23):8045-55
3)	Tous C, et al. (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
4)	Medler S, et al. (2011) Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping. J Biol Chem 286(39):33709-18
5)	Gordon J, et al. (2011) Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology. Biochemistry 50(47):10203-14
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41