HEM2/YGL040C Protein Information

Standard Name	Hem2p 1
Systematic Name	Ygl040cp
Alias	Slu1p 2 , Ole4p 3, 4
ORF Classification	Verified
Description	Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus (1, 3, 5, 6)
Name Description	HEMe biosynthesis 1, 3

Experimental Data
Molecules/cell	11600 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	342
Molecular Weight (Da)	37,740
Isoelectric Point (pI)	6.06

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hem2p (InterPro)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000112B17 \| P05373 MIPS: YGL040C NCBI: 11514397 \| 11514757 \| 1322524 \| 157830112 \| 15825885 \| 15825886 \| 15826267 \| 15826306 \| 15826422 \| 1708174 \| 171664 \| 17943449 \| 33357749 \| 51012939 \| 6321398 \| 7245674 \| 75765315 \| NP_011475.1 \| NM_001180905.1 GenBank/EMBL/DDBJ: DAA08060.1 \| AY692744 \| J03493 \| Z72562
External Classifications	EC: 4.2.1.24 [Porphobilinogen synthase]

Amino Acid Sequence (or in FASTA format)
1 MHTAEFLETE PTEISSVLAG GYNHPLLRQW QSERQLTKNM LIFPLFISDN 51 PDDFTEIDSL PNINRIGVNR LKDYLKPLVA KGLRSVILFG VPLIPGTKDP 101 VGTAADDPAG PVIQGIKFIR EYFPELYIIC DVCLCEYTSH GHCGVLYDDG 151 TINRERSVSR LAAVAVNYAK AGAHCVAPSD MIDGRIRDIK RGLINANLAH 201 KTFVLSYAAK FSGNLYGPFR DAACSAPSNG DRKCYQLPPA GRGLARRALE 251 RDMSEGADGI IVKPSTFYLD IMRDASEICK DLPICAYHVS GEYAMLHAAA 301 EKGVVDLKTI AFESHQGFLR AGARLIITYL APEFLDWLDE EN*

Amino Acid Sequence (or in FASTA format)

       1  MHTAEFLETE PTEISSVLAG GYNHPLLRQW QSERQLTKNM LIFPLFISDN
      51  PDDFTEIDSL PNINRIGVNR LKDYLKPLVA KGLRSVILFG VPLIPGTKDP
     101  VGTAADDPAG PVIQGIKFIR EYFPELYIIC DVCLCEYTSH GHCGVLYDDG
     151  TINRERSVSR LAAVAVNYAK AGAHCVAPSD MIDGRIRDIK RGLINANLAH
     201  KTFVLSYAAK FSGNLYGPFR DAACSAPSNG DRKCYQLPPA GRGLARRALE
     251  RDMSEGADGI IVKPSTFYLD IMRDASEICK DLPICAYHVS GEYAMLHAAA
     301  EKGVVDLKTI AFESHQGFLR AGARLIITYL APEFLDWLDE EN*

external links for Hem2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Hem2p

1)	Myers AM, et al. (1987) Characterization of the yeast HEM2 gene and transcriptional regulation of COX5 and COR1 by heme. J Biol Chem 262(35):16822-9
2)	Gachotte D, et al. (1997) A yeast sterol auxotroph (erg25) is rescued by addition of azole antifungals and reduced levels of heme. Proc Natl Acad Sci U S A 94(21):11173-8
3)	Gollub EG, et al. (1977) Yeast mutants deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3-oxidosqualene. J Biol Chem 252(9):2846-54
4)	Bard M (1972) Biochemical and genetic aspects of nystatin resistance in saccharomyces cerevisiae. J Bacteriol 111(3):649-57
5)	Borralho LM, et al. (1990) Purification of delta-aminolevulinate dehydratase from genetically engineered yeast. Yeast 6(4):319-30
6)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41