PNC1/YGL037C Protein Information

Standard Name	Pnc1p 1
Systematic Name	Ygl037cp
ORF Classification	Verified
Description	Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress (1, 2, 3)
Name Description	Pyrazinamidase and NiCotinamidase 1

Experimental Data
Molecules/cell	7720 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	216
Molecular Weight (Da)	24,993
Isoelectric Point (pI)	6.23

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pnc1p (InterPro)
Physical Interactions	There are 20 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B04C \| P53184 MIPS: YGL037C NCBI: 1322518 \| 145579476 \| 145579477 \| 145579478 \| 145579479 \| 145579480 \| 145579481 \| 145579482 \| 1723821 \| 374074558 \| 374074559 \| 374074560 \| 374074561 \| 374074562 \| 374074563 \| 374074564 \| 398364807 \| 45270852 \| NP_011478.3 GenBank/EMBL/DDBJ: DAA08063.1 \| AY558481 \| Z72559
External Classifications	EC: 3.5.1.19 [Nicotinamidase]

Amino Acid Sequence (or in FASTA format)
1 MKTLIVVDMQ NDFISPLGSL TVPKGEELIN PISDLMQDAD RDWHRIVVTR 51 DWHPSRHISF AKNHKDKEPY STYTYHSPRP GDDSTQEGIL WPVHCVKNTW 101 GSQLVDQIMD QVVTKHIKIV DKGFLTDREY YSAFHDIWNF HKTDMNKYLE 151 KHHTDEVYIV GVALEYCVKA TAISAAELGY KTTVLLDYTR PISDDPEVIN 201 KVKEELKAHN INVVDK*

Amino Acid Sequence (or in FASTA format)

       1  MKTLIVVDMQ NDFISPLGSL TVPKGEELIN PISDLMQDAD RDWHRIVVTR
      51  DWHPSRHISF AKNHKDKEPY STYTYHSPRP GDDSTQEGIL WPVHCVKNTW
     101  GSQLVDQIMD QVVTKHIKIV DKGFLTDREY YSAFHDIWNF HKTDMNKYLE
     151  KHHTDEVYIV GVALEYCVKA TAISAAELGY KTTVLLDYTR PISDDPEVIN
     201  KVKEELKAHN INVVDK*

external links for Pnc1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pnc1p

1)	Ghislain M, et al. (2002) Identification and functional analysis of the Saccharomyces cerevisiae nicotinamidase gene, PNC1. Yeast 19(3):215-24
2)	Anderson RM, et al. (2003) Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae. Nature 423(6936):181-5
3)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41