PGD1/YGL025C Protein Information

Standard Name	Pgd1p 1
Systematic Name	Ygl025cp
Alias	Hrs1p , Med3p
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor (2, 3, 4, 5, 6)
Name Description	PolyGlutamine Domain 1

Experimental Data
Molecules/cell	556 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	397
Molecular Weight (Da)	43,079
Isoelectric Point (pI)	9.13

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pgd1p (InterPro)
Physical Interactions	There are 155 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001D4782 \| P40356 MIPS: YGL025C NCBI: 1322493 \| 37362652 \| 51013385 \| 531546 \| 780277 \| 78099785 \| NP_011490.2 GenBank/EMBL/DDBJ: DAA08074.1 \| AY692967 \| X81457 \| Z26254 \| Z72547

Amino Acid Sequence (or in FASTA format)
1 MDSIIPAGVK LDDLQVILAK NENETRDKVC KQINEARDEI LPLRLQFNEF 51 IQIMANIDQE GSKQADRMAK YLHIRDKILQ LNDRFQTLSS HLEALQPLFS 101 TVPEYLKTAD NRDRSFQLLE PLSTYNKNGN AVCSTATVVS TNHSAAASTP 151 TTTATPHANP ITHAHSLSNP NSTATMQHNP LAGKRGPKSG STMGTPTVHN 201 STAAAPIAAP KKPRKPRQTK KAKAQAQAQA QAQAQVYAQQ STVQTPITAS 251 MAAALPNPTP SMINSVSPTN VMGTPLTNMM SPMGNAYSMG AQNQGGQVSM 301 SQFNGSGNGS NPNTNTNSNN TPLQSQLNLN NLTPANILNM SMNNDFQQQQ 351 QQQQQQQQPQ PQYNMNMGMN NMNNGGKELD SLDLNNLELG GLNMDFL*

Amino Acid Sequence (or in FASTA format)

       1  MDSIIPAGVK LDDLQVILAK NENETRDKVC KQINEARDEI LPLRLQFNEF
      51  IQIMANIDQE GSKQADRMAK YLHIRDKILQ LNDRFQTLSS HLEALQPLFS
     101  TVPEYLKTAD NRDRSFQLLE PLSTYNKNGN AVCSTATVVS TNHSAAASTP
     151  TTTATPHANP ITHAHSLSNP NSTATMQHNP LAGKRGPKSG STMGTPTVHN
     201  STAAAPIAAP KKPRKPRQTK KAKAQAQAQA QAQAQVYAQQ STVQTPITAS
     251  MAAALPNPTP SMINSVSPTN VMGTPLTNMM SPMGNAYSMG AQNQGGQVSM
     301  SQFNGSGNGS NPNTNTNSNN TPLQSQLNLN NLTPANILNM SMNNDFQQQQ
     351  QQQQQQQQPQ PQYNMNMGMN NMNNGGKELD SLDLNNLELG GLNMDFL*

external links for Pgd1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Pgd1p

1)	Brohl S, et al. (1994) A new nuclear suppressor system for a mitochondrial RNA polymerase mutant identifies an unusual zinc-finger protein and a polyglutamine domain protein in Saccharomyces cerevisiae. Yeast 10(6):719-31
2)	Papamichos-Chronakis M, et al. (2000) Hrs1/Med3 is a Cyc8-Tup1 corepressor target in the RNA polymerase II holoenzyme. J Biol Chem 275(12):8397-403
3)	Lee M, et al. (2000) Genetic analysis of the role of Pol II holoenzyme components in repression by the Cyc8-Tup1 corepressor in yeast. Genetics 155(4):1535-42
4)	Reeves WM and Hahn S (2003) Activator-independent functions of the yeast mediator sin4 complex in preinitiation complex formation and transcription reinitiation. Mol Cell Biol 23(1):349-58
5)	Myers LC, et al. (1999) Mediator protein mutations that selectively abolish activated transcription. Proc Natl Acad Sci U S A 96(1):67-72
6)	Guglielmi B, et al. (2004) A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32(18):5379-91
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41