PUF4/YGL014W Protein Information

Standard Name	Puf4p 1
Systematic Name	Ygl014wp
Alias	Ygl023p 2
ORF Classification	Verified
Description	Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors (3)
Name Description	PUmilio-homology domain Family

Experimental Data
Molecules/cell	721 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	888
Molecular Weight (Da)	97,797
Isoelectric Point (pI)	6.59

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Puf4p (InterPro)
Physical Interactions	There are 67 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B020 \| P25339 MIPS: YGL014W NCBI: 1322473 \| 169791859 \| 169791860 \| 169791861 \| 169791866 \| 169791867 \| 1723801 \| 234327 \| 385252070 \| 385252071 \| 4261598 \| 6321424 \| NP_011501.1 GenBank/EMBL/DDBJ: DAA08084.1 \| S57889 \| S58126 \| Z72536

Amino Acid Sequence (or in FASTA format)
1 MSTKGLKEEI DDVPSVDPVV SETVNSALEQ LQLDDPEENA TSNAFANKVS 51 QDSQFANGPP SQMFPHPQMM GGMGFMPYSQ MMQVPHNPCP FFPPPDFNDP 101 TAPLSSSPLN AGGPPMLFKN DSLPFQMLSS GAAVATQGGQ NLNPLINDNS 151 MKVLPIASAD PLWTHSNVPG SASVAIEETT ATLQESLPSK GRESNNKASS 201 FRRQTFHALS PTDLINAANN VTLSKDFQSD MQNFSKAKKP SVGANNTAKT 251 RTQSISFDNT PSSTSFIPPT NSVSEKLSDF KIETSKEDLI NKTAPAKKES 301 PTTYGAAYPY GGPLLQPNPI MPGHPHNISS PIYGIRSPFP NSYEMGAQFQ 351 PFSPILNPTS HSLNANSPIP LTQSPIHLAP VLNPSSNSVA FSDMKNDGGK 401 PTTDNDKAGP NVRMDLINPN LGPSMQPFHI LPPQQNTPPP PWLYSTPPPF 451 NAMVPPHLLA QNHMPLMNSA NNKHHGRNNN SMSSHNDNDN IGNSNYNNKD 501 TGRSNVGKMK NMKNSYHGYY NNNNNNNNNN NNNNNSNATN SNSAEKQRKI 551 EESSRFADAV LDQYIGSIHS LCKDQHGCRF LQKQLDILGS KAADAIFEET 601 KDYTVELMTD SFGNYLIQKL LEEVTTEQRI VLTKISSPHF VEISLNPHGT 651 RALQKLIECI KTDEEAQIVV DSLRPYTVQL SKDLNGNHVI QKCLQRLKPE 701 NFQFIFDAIS DSCIDIATHR HGCCVLQRCL DHGTTEQCDN LCDKLLALVD 751 KLTLDPFGNY VVQYIITKEA EKNKYDYTHK IVHLLKPRAI ELSIHKFGSN 801 VIEKILKTAI VSEPMILEIL NNGGETGIQS LLNDSYGNYV LQTALDISHK 851 QNDYLYKRLS EIVAPLLVGP IRNTPHGKRI IGMLHLDS*

Amino Acid Sequence (or in FASTA format)

       1  MSTKGLKEEI DDVPSVDPVV SETVNSALEQ LQLDDPEENA TSNAFANKVS
      51  QDSQFANGPP SQMFPHPQMM GGMGFMPYSQ MMQVPHNPCP FFPPPDFNDP
     101  TAPLSSSPLN AGGPPMLFKN DSLPFQMLSS GAAVATQGGQ NLNPLINDNS
     151  MKVLPIASAD PLWTHSNVPG SASVAIEETT ATLQESLPSK GRESNNKASS
     201  FRRQTFHALS PTDLINAANN VTLSKDFQSD MQNFSKAKKP SVGANNTAKT
     251  RTQSISFDNT PSSTSFIPPT NSVSEKLSDF KIETSKEDLI NKTAPAKKES
     301  PTTYGAAYPY GGPLLQPNPI MPGHPHNISS PIYGIRSPFP NSYEMGAQFQ
     351  PFSPILNPTS HSLNANSPIP LTQSPIHLAP VLNPSSNSVA FSDMKNDGGK
     401  PTTDNDKAGP NVRMDLINPN LGPSMQPFHI LPPQQNTPPP PWLYSTPPPF
     451  NAMVPPHLLA QNHMPLMNSA NNKHHGRNNN SMSSHNDNDN IGNSNYNNKD
     501  TGRSNVGKMK NMKNSYHGYY NNNNNNNNNN NNNNNSNATN SNSAEKQRKI
     551  EESSRFADAV LDQYIGSIHS LCKDQHGCRF LQKQLDILGS KAADAIFEET
     601  KDYTVELMTD SFGNYLIQKL LEEVTTEQRI VLTKISSPHF VEISLNPHGT
     651  RALQKLIECI KTDEEAQIVV DSLRPYTVQL SKDLNGNHVI QKCLQRLKPE
     701  NFQFIFDAIS DSCIDIATHR HGCCVLQRCL DHGTTEQCDN LCDKLLALVD
     751  KLTLDPFGNY VVQYIITKEA EKNKYDYTHK IVHLLKPRAI ELSIHKFGSN
     801  VIEKILKTAI VSEPMILEIL NNGGETGIQS LLNDSYGNYV LQTALDISHK
     851  QNDYLYKRLS EIVAPLLVGP IRNTPHGKRI IGMLHLDS*

external links for Puf4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Puf4p

1)	Olivas W and Parker R (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11
2)	Chen WN, et al. (1991) The YGL023 gene encodes a putative regulatory protein. Yeast 7(3):309-12
3)	Gerber AP, et al. (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41