LEU1/YGL009C Protein Information

Standard Name	Leu1p
Systematic Name	Ygl009cp
ORF Classification	Verified
Description	Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway (1, 2)
Name Description	LEUcine biosynthesis 1

Experimental Data
Molecules/cell	96300 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	779
Molecular Weight (Da)	85,794
Isoelectric Point (pI)	5.74

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Leu1p (InterPro)
Physical Interactions	There are 23 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053278 \| P07264 MIPS: YGL009C NCBI: 1322463 \| 1708801 \| 171835 \| 234318 \| 6321429 \| NP_011506.1 GenBank/EMBL/DDBJ: DAA08089.1 \| K01969 \| S57886 \| S58126 \| Z72531
External Classifications	EC: 4.2.1.33 [3-isopropylmalate dehydratase]

Amino Acid Sequence (or in FASTA format)
1 MVYTPSKGPR TLYDKVFDAH VVHQDENGSF LLYIDRHLVH EVTSPQAFEG 51 LENAGRKVRR VDCTLATVDH NIPTESRKNF KSLDTFIKQT DSRLQVKTLE 101 NNVKQFGVPY FGMSDARQGI VHTIGPEEGF TLPGTTVVCG DSHTSTHGAF 151 GSLAFGIGTS EVEHVLATQT IIQAKSKNMR ITVNGKLSPG ITSKDLILYI 201 IGLIGTAGGT GCVIEFAGEA IEALSMEARM SMCNMAIEAG ARAGMIKPDE 251 TTFQYTKGRP LAPKGAEWEK AVAYWKTLKT DEGAKFDHEI NIEAVDVIPT 301 ITWGTSPQDA LPITGSVPDP KNVTDPIKKS GMERALAYMG LEPNTPLKSI 351 KVDKVFIGSC TNGRIEDLRS AAAVVRGQKL ASNIKLAMVV PGSGLVKKQA 401 EAEGLDKIFQ EAGFEWREAG CSICLGMNPD ILDAYERCAS TSNRNFEGRQ 451 GALSRTHLMS PAMAAAAGIA GHFVDIREFE YKDQDQSSPK VEVTSEDEKE 501 LESAAYDHAE PVQPEDAPQD IANDELKDIP VKSDDTPAKP SSSGMKPFLT 551 LEGISAPLDK ANVDTDAIIP KQFLKTIKRT GLKKGLFYEW RFRKDDQGKD 601 QETDFVLNVE PWREAEILVV TGDNFGCGSS REHAPWALKD FGIKSIIAPS 651 YGDIFYNNSF KNGLLPIRLD QQIIIDKLIP IANKGGKLCV DLPNQKILDS 701 DGNVLVDHFE IEPFRKHCLV NGLDDIGITL QKEEYISRYE ALRREKYSFL 751 EGGSKLLKFD NVPKRKAVTT TFDKVHQDW*

Amino Acid Sequence (or in FASTA format)

       1  MVYTPSKGPR TLYDKVFDAH VVHQDENGSF LLYIDRHLVH EVTSPQAFEG
      51  LENAGRKVRR VDCTLATVDH NIPTESRKNF KSLDTFIKQT DSRLQVKTLE
     101  NNVKQFGVPY FGMSDARQGI VHTIGPEEGF TLPGTTVVCG DSHTSTHGAF
     151  GSLAFGIGTS EVEHVLATQT IIQAKSKNMR ITVNGKLSPG ITSKDLILYI
     201  IGLIGTAGGT GCVIEFAGEA IEALSMEARM SMCNMAIEAG ARAGMIKPDE
     251  TTFQYTKGRP LAPKGAEWEK AVAYWKTLKT DEGAKFDHEI NIEAVDVIPT
     301  ITWGTSPQDA LPITGSVPDP KNVTDPIKKS GMERALAYMG LEPNTPLKSI
     351  KVDKVFIGSC TNGRIEDLRS AAAVVRGQKL ASNIKLAMVV PGSGLVKKQA
     401  EAEGLDKIFQ EAGFEWREAG CSICLGMNPD ILDAYERCAS TSNRNFEGRQ
     451  GALSRTHLMS PAMAAAAGIA GHFVDIREFE YKDQDQSSPK VEVTSEDEKE
     501  LESAAYDHAE PVQPEDAPQD IANDELKDIP VKSDDTPAKP SSSGMKPFLT
     551  LEGISAPLDK ANVDTDAIIP KQFLKTIKRT GLKKGLFYEW RFRKDDQGKD
     601  QETDFVLNVE PWREAEILVV TGDNFGCGSS REHAPWALKD FGIKSIIAPS
     651  YGDIFYNNSF KNGLLPIRLD QQIIIDKLIP IANKGGKLCV DLPNQKILDS
     701  DGNVLVDHFE IEPFRKHCLV NGLDDIGITL QKEEYISRYE ALRREKYSFL
     751  EGGSKLLKFD NVPKRKAVTT TFDKVHQDW*

external links for Leu1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Leu1p

1)	Hsu YP and Schimmel P (1984) Yeast LEU1. Repression of mRNA levels by leucine and relationship of 5'-noncoding region to that of LEU2. J Biol Chem 259(6):3714-9
2)	Skala J, et al. (1991) Complete sequence of the Saccharomyces cerevisiae LEU1 gene encoding isopropylmalate isomerase. Yeast 7(3):281-5
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41