RPN14/YGL004C Protein Information

Standard Name	Rpn14p 1
Systematic Name	Ygl004cp
ORF Classification	Verified
Description	Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p (1, 2, 3, 4, 5, 6)
Name Description	Regulatory Particle Non-ATPase 1

Experimental Data
Molecules/cell	2210 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	417
Molecular Weight (Da)	46,383
Isoelectric Point (pI)	4.94

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rpn14p (InterPro)
Physical Interactions	There are 58 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B00A \| P53196 MIPS: YGL004C NCBI: 1322453 \| 1723796 \| 291191089 \| 385867657 \| 385867658 \| 398364993 \| 51012649 \| NP_011511.3 GenBank/EMBL/DDBJ: DAA08095.1 \| AY692599 \| Z72526

Amino Acid Sequence (or in FASTA format)
1 MTKTITVAHI QYDFKAVLEE NDENDDEFYI NVDKNLNEIK EHKIVVLGNS 51 RGVDAGKGNT FEKVGSHLYK ARLDGHDFLF NTIIRDGSKM LKRADYTAVD 101 TAKLQMRRFI LGTTEGDIKV LDSNFNLQRE IDQAHVSEIT KLKFFPSGEA 151 LISSSQDMQL KIWSVKDGSN PRTLIGHRAT VTDIAIIDRG RNVLSASLDG 201 TIRLWECGTG TTIHTFNRKE NPHDGVNSIA LFVGTDRQLH EISTSKKNNL 251 EFGTYGKYVI AGHVSGVITV HNVFSKEQTI QLPSKFTCSC NSLTVDGNNA 301 NYIYAGYENG MLAQWDLRSP ECPVGEFLIN EGTPINNVYF AAGALFVSSG 351 FDTSIKLDII SDPESERPAI EFETPTFLVS NDDEVSQFCY VSDDESNGEV 401 LEVGKNNFCA LYNLSNP*

Amino Acid Sequence (or in FASTA format)

       1  MTKTITVAHI QYDFKAVLEE NDENDDEFYI NVDKNLNEIK EHKIVVLGNS
      51  RGVDAGKGNT FEKVGSHLYK ARLDGHDFLF NTIIRDGSKM LKRADYTAVD
     101  TAKLQMRRFI LGTTEGDIKV LDSNFNLQRE IDQAHVSEIT KLKFFPSGEA
     151  LISSSQDMQL KIWSVKDGSN PRTLIGHRAT VTDIAIIDRG RNVLSASLDG
     201  TIRLWECGTG TTIHTFNRKE NPHDGVNSIA LFVGTDRQLH EISTSKKNNL
     251  EFGTYGKYVI AGHVSGVITV HNVFSKEQTI QLPSKFTCSC NSLTVDGNNA
     301  NYIYAGYENG MLAQWDLRSP ECPVGEFLIN EGTPINNVYF AAGALFVSSG
     351  FDTSIKLDII SDPESERPAI EFETPTFLVS NDDEVSQFCY VSDDESNGEV
     401  LEVGKNNFCA LYNLSNP*

external links for Rpn14p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rpn14p

1)	Samanta MP and Liang S (2003) Predicting protein functions from redundancies in large-scale protein interaction networks. Proc Natl Acad Sci U S A 100(22):12579-83
2)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3)	Seong KM, et al. (2007) Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. FEBS Lett 581(13):2567-73
4)	Roelofs J, et al. (2009) Chaperone-mediated pathway of proteasome regulatory particle assembly. Nature 459(7248):861-5
5)	Saeki Y, et al. (2009) Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle. Cell 137(5):900-13
6)	Funakoshi M, et al. (2009) Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base. Cell 137(5):887-99
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41