YRF1-1/YDR545W Protein Information

Standard Name	Yrf1-1p
Systematic Name	Ydr545wp
Alias	Yrf1p
ORF Classification	Verified
Description	Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p (1, 2)

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,796
Molecular Weight (Da)	203,779
Isoelectric Point (pI)	7.49

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yrf1-1p (InterPro)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F904 \| P0CX20 MIPS: YDR545W NCBI: 1165300 \| 1431574 \| 338819800 \| 398366399 \| 398366465 \| 6320754 \| NP_010834.1 \| NP_013572.3 \| NP_061495.4 GenBank/EMBL/DDBJ: DAA12372.1 \| U43834 \| Z74389
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.12 [DNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MKVSDRRKFE KANFDEFESA LNNKNDLVHC PSITLFESIP TEVRSFYEDE 51 KSGLIKVVKF RTGAMDRKRS FEKIVISVMV GKNVQKFLTF VEDEPDFQGG 101 PIPSKYLIPK KINLMVYTLF QVHTLKFNRK DYDTLSLFYL NRGYYNELSF 151 RVLERCHEIA SARPNDSSTM RTFTDFVSGA PIVRSLQKST IRKYGYNLAP 201 YMFLLLHVDE LSIFSAYQAS LPGEKKVDTE RLKRDLCPRK PIEIKYFSQI 251 CNDMMNKKDR LGDILHIILR ACALNFGAGP RGGAGDEEDR SITNEEPIIP 301 SVDEHGLKVC KLRSPNTPRR LRKTLDAVKA LLVSSCACTA RDLDIFDDTN 351 GVAMWKWIKI LYHEVAQETT LKDSYRITLV PSSDGISVCG KLFNREYVRG 401 FYFACKAQFD NLWGELNNCF YMPTVVDIAS LILRNREVLF REPKRGIDEY 451 LENDSFLQMI PVKYREIVLP KLRRDTNKMT AALKNKVTVA IDELTVPLMW 501 MVHFAVGYPY RYPELQLLAF AGPQRNVYVD DTTRRIQLYT DYNKNGSSEP 551 RLKTLDGLTS DYVFYFVTVL RQMQICALGN SYDAFNHDPW MDVVGFEDPD 601 QVTNRDISRI VLYSYMFLNT AKGCLVEYAT FRQYMRELPK NAPQKLNFRE 651 MRQGLIALGR HCVGSRFETD LYESATSELM ANHSVQTGRN IYGVDSFSLT 701 SVSGTTATLL QERASERWIQ WLGLESDYHC SFSSTRNAED VVAGEAASSD 751 HDQKISRVTR KRPREPKSTN DILVAGQKLF GSSFEFRDLH QLRLCHEIYM 801 ADTPSVAVQA PPGYGKTELF HLPLIALASK GDVKYVSFLF VPYTVLLANC 851 MIRLSRCGCL NVAPVRNFIE EGCDGVTDLY VGIYDDLAST NFTDRIAAWE 901 NIVECTFRTN NVKLGYLIVD EFHNFETEVY RQSQFGGITN LDFDAFEKAI 951 FLSGTAPEAV ADAALQRIGL TGLAKKSMDI NELKRSEDLS RGLSSYPTRM 1001 FNLIKEKSEV PLGHVHKIWK KVESQPEEAL KLLLALFEIE PESKAIVVAS 1051 TTNEVEELAC SWRKYFRVVW IHGKLGAAEK VSRTKEFVTD GSMRVLIGTK 1101 LVTEGIDIKQ LMMVIMLDNR LNIIELIQGV GRLRDGGLCY LLSRKNSWAA 1151 RNRKGELPPI KEGCITEQVR EFYGLESKKG KKGQHVGCCG SRTDLSADTV 1201 ELIERMDRLA EKQATASMSI IALPSSFQES NSSDRCRKYC SSDEDSDTCI 1251 HGSANASTNA TTNSSTNATT TASTNVRTSA TTTASINVRT SAITTESTNS 1301 STNATTTAST NVRTSATTTA SINVRTSATT TESTNSNTSA TTTESTDSNT 1351 SATTTESTDS NTSATTTAST NSSTNATTTA STNSSTNATT TESTNASAKE 1401 DANKDGNAED NRFHPVTDIN KESYKRKGSQ MVLLERKKLK AQFPNTSENM 1451 NVLQFLGFRS DEIKHLFLYG IDVYFCPEGV FTQYGLCKGC QKMFELCVCW 1501 AGQKVSYRRM AWEALAVERM LRNDEEYKEY LEDIEPYHGD PVGYLKYFSV 1551 KRGEIYSQIQ RNYAWYLAIT RRRETISVLD STRGKQGSQV FRMSGRQIKE 1601 LYYKVWSNLR ESKTEVLQYF LNWDEKKCRE EWEAKDDTVF VEALEKVGVF 1651 QRLRSMTSAG LQGPQYVKLQ FSRHHRQLRS RYELSLGMHL RDQLALGVTP 1701 SKVPHWTAFL SMLIGLFYNK TFRQKLEYLL EQISEVWLLP HWLDLANVEV 1751 LAADNTRVPL YMLMVAVHKE LDSDDVPDGR FDIILLCRDS SREVGE*

Amino Acid Sequence (or in FASTA format)

       1  MKVSDRRKFE KANFDEFESA LNNKNDLVHC PSITLFESIP TEVRSFYEDE
      51  KSGLIKVVKF RTGAMDRKRS FEKIVISVMV GKNVQKFLTF VEDEPDFQGG
     101  PIPSKYLIPK KINLMVYTLF QVHTLKFNRK DYDTLSLFYL NRGYYNELSF
     151  RVLERCHEIA SARPNDSSTM RTFTDFVSGA PIVRSLQKST IRKYGYNLAP
     201  YMFLLLHVDE LSIFSAYQAS LPGEKKVDTE RLKRDLCPRK PIEIKYFSQI
     251  CNDMMNKKDR LGDILHIILR ACALNFGAGP RGGAGDEEDR SITNEEPIIP
     301  SVDEHGLKVC KLRSPNTPRR LRKTLDAVKA LLVSSCACTA RDLDIFDDTN
     351  GVAMWKWIKI LYHEVAQETT LKDSYRITLV PSSDGISVCG KLFNREYVRG
     401  FYFACKAQFD NLWGELNNCF YMPTVVDIAS LILRNREVLF REPKRGIDEY
     451  LENDSFLQMI PVKYREIVLP KLRRDTNKMT AALKNKVTVA IDELTVPLMW
     501  MVHFAVGYPY RYPELQLLAF AGPQRNVYVD DTTRRIQLYT DYNKNGSSEP
     551  RLKTLDGLTS DYVFYFVTVL RQMQICALGN SYDAFNHDPW MDVVGFEDPD
     601  QVTNRDISRI VLYSYMFLNT AKGCLVEYAT FRQYMRELPK NAPQKLNFRE
     651  MRQGLIALGR HCVGSRFETD LYESATSELM ANHSVQTGRN IYGVDSFSLT
     701  SVSGTTATLL QERASERWIQ WLGLESDYHC SFSSTRNAED VVAGEAASSD
     751  HDQKISRVTR KRPREPKSTN DILVAGQKLF GSSFEFRDLH QLRLCHEIYM
     801  ADTPSVAVQA PPGYGKTELF HLPLIALASK GDVKYVSFLF VPYTVLLANC
     851  MIRLSRCGCL NVAPVRNFIE EGCDGVTDLY VGIYDDLAST NFTDRIAAWE
     901  NIVECTFRTN NVKLGYLIVD EFHNFETEVY RQSQFGGITN LDFDAFEKAI
     951  FLSGTAPEAV ADAALQRIGL TGLAKKSMDI NELKRSEDLS RGLSSYPTRM
    1001  FNLIKEKSEV PLGHVHKIWK KVESQPEEAL KLLLALFEIE PESKAIVVAS
    1051  TTNEVEELAC SWRKYFRVVW IHGKLGAAEK VSRTKEFVTD GSMRVLIGTK
    1101  LVTEGIDIKQ LMMVIMLDNR LNIIELIQGV GRLRDGGLCY LLSRKNSWAA
    1151  RNRKGELPPI KEGCITEQVR EFYGLESKKG KKGQHVGCCG SRTDLSADTV
    1201  ELIERMDRLA EKQATASMSI IALPSSFQES NSSDRCRKYC SSDEDSDTCI
    1251  HGSANASTNA TTNSSTNATT TASTNVRTSA TTTASINVRT SAITTESTNS
    1301  STNATTTAST NVRTSATTTA SINVRTSATT TESTNSNTSA TTTESTDSNT
    1351  SATTTESTDS NTSATTTAST NSSTNATTTA STNSSTNATT TESTNASAKE
    1401  DANKDGNAED NRFHPVTDIN KESYKRKGSQ MVLLERKKLK AQFPNTSENM
    1451  NVLQFLGFRS DEIKHLFLYG IDVYFCPEGV FTQYGLCKGC QKMFELCVCW
    1501  AGQKVSYRRM AWEALAVERM LRNDEEYKEY LEDIEPYHGD PVGYLKYFSV
    1551  KRGEIYSQIQ RNYAWYLAIT RRRETISVLD STRGKQGSQV FRMSGRQIKE
    1601  LYYKVWSNLR ESKTEVLQYF LNWDEKKCRE EWEAKDDTVF VEALEKVGVF
    1651  QRLRSMTSAG LQGPQYVKLQ FSRHHRQLRS RYELSLGMHL RDQLALGVTP
    1701  SKVPHWTAFL SMLIGLFYNK TFRQKLEYLL EQISEVWLLP HWLDLANVEV
    1751  LAADNTRVPL YMLMVAVHKE LDSDDVPDGR FDIILLCRDS SREVGE*

external links for Yrf1-1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Yrf1-1p

1)	Yamada M, et al. (1998) Y'-Help1, a DNA helicase encoded by the yeast subtelomeric Y' element, is induced in survivors defective for telomerase. J Biol Chem 273(50):33360-6
2)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64