EMI2/YDR516C Protein Information

Standard Name	Emi2p 1
Systematic Name	Ydr516cp
ORF Classification	Verified
Description	Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description	Early Meiotic Induction 1

Experimental Data
Molecules/cell	10600 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	500
Molecular Weight (Da)	55,920
Isoelectric Point (pI)	6.22

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Emi2p (InterPro)
Physical Interactions	There are 15 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006C3DF \| Q04409 MIPS: YDR516C NCBI: 398366669 \| 73620870 \| 927785 \| NP_010804.3 GenBank/EMBL/DDBJ: DAA12347.1 \| U33057
External Classifications	EC: 2.7.1.2 [Glucokinase]

Amino Acid Sequence (or in FASTA format)
1 MSFENLHKVN AEALEDAVVE ICSSLQVDAA KLDELTAYFI ECMEKGLNNT 51 SVGEEKTVDK GLPMIPTYVT SLPNGTERGV LLAADLGGTH FRVCSVTLNG 101 DGTFDMQQLK SKIPEEYLND KDVTSEELFS YLGRRTRAFV RKHHPELLKS 151 TGENIKPLKM GFTFSYPVDQ TSLSSGTLIR WTKSFKIEDT VGKDVVRLYQ 201 EQLDIQGLSM INVVALTNDT VGTFLSHCYT SGSRPSSAGE ISEPVIGCIF 251 GTGTNGCYME DIENIKKLPD ELRTRLLHEG KTQMCINIEW GSFDNELKHL 301 SATKYDIDID QKFSPNPGYH LFEKRISGMY LGELLRNILV DLHARGLILG 351 QYRNYDQLPH RLKTPFQLCS EVLSRIEIDD STNLRETELS FLQSLRLPTT 401 FEERKAIQNL VRSITRRSAY LAAVPIAAIL IKTNALNKRY HGEVEIGFDG 451 YVIEYYPGFR SMLRHALALS PIGTEGERKI HLRLAKDGSG VGAALCALVA 501 *

Amino Acid Sequence (or in FASTA format)

       1  MSFENLHKVN AEALEDAVVE ICSSLQVDAA KLDELTAYFI ECMEKGLNNT
      51  SVGEEKTVDK GLPMIPTYVT SLPNGTERGV LLAADLGGTH FRVCSVTLNG
     101  DGTFDMQQLK SKIPEEYLND KDVTSEELFS YLGRRTRAFV RKHHPELLKS
     151  TGENIKPLKM GFTFSYPVDQ TSLSSGTLIR WTKSFKIEDT VGKDVVRLYQ
     201  EQLDIQGLSM INVVALTNDT VGTFLSHCYT SGSRPSSAGE ISEPVIGCIF
     251  GTGTNGCYME DIENIKKLPD ELRTRLLHEG KTQMCINIEW GSFDNELKHL
     301  SATKYDIDID QKFSPNPGYH LFEKRISGMY LGELLRNILV DLHARGLILG
     351  QYRNYDQLPH RLKTPFQLCS EVLSRIEIDD STNLRETELS FLQSLRLPTT
     401  FEERKAIQNL VRSITRRSAY LAAVPIAAIL IKTNALNKRY HGEVEIGFDG
     451  YVIEYYPGFR SMLRHALALS PIGTEGERKI HLRLAKDGSG VGAALCALVA
     501  *

external links for Emi2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Emi2p

1)	Enyenihi AH and Saunders WS (2003) Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae. Genetics 163(1):47-54
2)	Lutfiyya LL, et al. (1998) Characterization of three related glucose repressors and genes they regulate in Saccharomyces cerevisiae. Genetics 150(4):1377-91
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41