SMT3/YDR510W Protein Information Help

Standard Name Smt3p
Systematic Name Ydr510wp
ORF Classification Verified
Description Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Suppressor of Mif Two
Experimental Data
Molecules/cell 2940 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 101
Molecular Weight (Da) 11,597
Isoelectric Point (pI) 4.76

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Smt3p (InterPro)
Physical Interactions There are 1521 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001122F6 | Q12306
MIPS: YDR510W
NCBI: 149241626 | 149241627 | 20151196 | 2501450 | 315583596 | 332639826 | 332639827 | 356624718 | 357380689 | 357380691 | 358439977 | 359546195 | 372467172 | 378792499 | 378792501 | 378792503 | 378792506 | 399125172 | 399125173 | 399125220 | 399125221 | 400977597 | 400977598 | 400977599 | 400977600 | 403072257 | 403072258 | 45270238 | 6320718 | 8569260 | 881372 | 927779 | NP_010798.1
GenBank/EMBL/DDBJ: DAA12341.1 | AY558174 | U27233 | U33057
Amino Acid Sequence (or in FASTA format)
 
       1  MSDSEVNQEA KPEVKPEVKP ETHINLKVSD GSSEIFFKIK KTTPLRRLME
      51  AFAKRQGKEM DSLRFLYDGI RIQADQTPED LDMEDNDIIE AHREQIGGAT
     101  Y*

external links for Smt3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Smt3p
1) Johnson ES and Blobel G  (1999) Cell cycle-regulated attachment of the ubiquitin-related protein SUMO to the yeast septins. J Cell Biol 147(5):981-94
2) Takahashi Y, et al.  (1999) Smt3, a SUMO-1 homolog, is conjugated to Cdc3, a component of septin rings at the mother-bud neck in budding yeast. Biochem Biophys Res Commun 259(3):582-7
3) Biggins S, et al.  (2001) Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae. Genetics 159(2):453-70
4) Bachant J, et al.  (2002) The SUMO-1 isopeptidase Smt4 is linked to centromeric cohesion through SUMO-1 modification of DNA topoisomerase II. Mol Cell 9(6):1169-82
5) Hoege C, et al.  (2002) RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO. Nature 419(6903):135-41
6) Dieckhoff P, et al.  (2004) Smt3/SUMO and Ubc9 are required for efficient APC/C-mediated proteolysis in budding yeast. Mol Microbiol 51(5):1375-87
7) Stead K, et al.  (2003) Pds5p regulates the maintenance of sister chromatid cohesion and is sumoylated to promote the dissolution of cohesion. J Cell Biol 163(4):729-41
8) Matic I, et al.  (2008) Phosphorylation of SUMO-1 Occurs in Vivo and Is Conserved through Evolution. J Proteome Res 7(9):4050-4057
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41