PUF6/YDR496C Protein Information

Standard Name	Puf6p
Systematic Name	Ydr496cp
ORF Classification	Verified
Description	Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis (1, 2)
Name Description	PUmilio-homology domain Family

Experimental Data
Molecules/cell	21500 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	656
Molecular Weight (Da)	75,105
Isoelectric Point (pI)	6.88

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Puf6p (InterPro)
Physical Interactions	There are 155 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052ED1 \| Q04373 MIPS: YDR496C NCBI: 34922920 \| 6320704 \| 927766 \| NP_010784.1 \| NM_001180804.1 GenBank/EMBL/DDBJ: DAA12328.1 \| U33057

Amino Acid Sequence (or in FASTA format)
1 MAPLTKKTNG KRSAKEVSHS EKKLAKKPRI SIDSSDEESE LSKKEDAVSS 51 SSDDDDLDDL STSDSEAEEE ADELDISDDS EEHENENEEK EGKDKSEGGE 101 NGNHTEQRKL LKERKMQRKS GTQVQQIKSV WERLRVKTPP LPKQIREKLS 151 NEIWELSKDC ISDLVLKHDA SRIVQTLVKY SSKDRREQIV DALKGKFYVL 201 ATSAYGKYLL VKLLHYGSRS SRQTIINELH GSLRKLMRHR EGAYVVEDLF 251 VLYATHEQRQ QMIKEFWGSE YAVFRETHKD LTIEKVCESS IEKRNIIARN 301 LIGTITASVE KGSTGFQILH AAMREYVKIA NEKEISEMIE LLHEQFAELV 351 HTPEGSDVAC TLVARANAKE RKLILKALKN HAEKLIKNEY GNIVFITILN 401 CVDDTVLVFK TFSPTVKEHL QEFIIDKFGR RPWLYILLGL DGKYFSPIVK 451 NELLRYIELS KATSKKDPLQ RRHELLSKFA PMFLSTISKD YSSILTENLG 501 CQFIAEVLIN DELYAQLNEK DQEKYQQVLN NILTTFKGDI TEEEHPIHRA 551 FSTRLLKALI QGGKWNNKEK KVIPLKNVQG LGVPFAEKLY DEIIDSSNLL 601 EWINNADSSF TIVALYETLK DQKEGKPFLK DLRGVQSKIT TDESNKGSQL 651 LAKLLK*

Amino Acid Sequence (or in FASTA format)

       1  MAPLTKKTNG KRSAKEVSHS EKKLAKKPRI SIDSSDEESE LSKKEDAVSS
      51  SSDDDDLDDL STSDSEAEEE ADELDISDDS EEHENENEEK EGKDKSEGGE
     101  NGNHTEQRKL LKERKMQRKS GTQVQQIKSV WERLRVKTPP LPKQIREKLS
     151  NEIWELSKDC ISDLVLKHDA SRIVQTLVKY SSKDRREQIV DALKGKFYVL
     201  ATSAYGKYLL VKLLHYGSRS SRQTIINELH GSLRKLMRHR EGAYVVEDLF
     251  VLYATHEQRQ QMIKEFWGSE YAVFRETHKD LTIEKVCESS IEKRNIIARN
     301  LIGTITASVE KGSTGFQILH AAMREYVKIA NEKEISEMIE LLHEQFAELV
     351  HTPEGSDVAC TLVARANAKE RKLILKALKN HAEKLIKNEY GNIVFITILN
     401  CVDDTVLVFK TFSPTVKEHL QEFIIDKFGR RPWLYILLGL DGKYFSPIVK
     451  NELLRYIELS KATSKKDPLQ RRHELLSKFA PMFLSTISKD YSSILTENLG
     501  CQFIAEVLIN DELYAQLNEK DQEKYQQVLN NILTTFKGDI TEEEHPIHRA
     551  FSTRLLKALI QGGKWNNKEK KVIPLKNVQG LGVPFAEKLY DEIIDSSNLL
     601  EWINNADSSF TIVALYETLK DQKEGKPFLK DLRGVQSKIT TDESNKGSQL
     651  LAKLLK*

external links for Puf6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Puf6p

1)	Gu W, et al. (2004) A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization. Genes Dev 18(12):1452-65
2)	Li Z, et al. (2009) Rational extension of the ribosome biogenesis pathway using network-guided genetics. PLoS Biol 7(10):e1000213
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41