PKH1/YDR490C Protein Information Help

Standard Name Pkh1p 1
Systematic Name Ydr490cp
ORF Classification Verified
Description Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Pkb-activating Kinase Homolog 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 766
Molecular Weight (Da) 86,252
Isoelectric Point (pI) 10.02

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pkh1p (InterPro)
Physical Interactions There are 48 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006A3C7 | Q03407
MIPS: YDR490C
NCBI: 398366645 | 51701748 | 927745 | NP_010778.3
GenBank/EMBL/DDBJ: DAA12323.1 | U33050
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MGNRSLTEAD HALLSKPLVP TSAEHTQTQE YPRPFVDGSN SQSGSELQAS
      51  PQGQFGEKAL TSTNRFIPLA NDDPGMQHEM GLDPSMRRRR EEWAERGAAK
     101  IVKDVVDPAT GELTKHVVKM GIKDFKFGEQ LGDGSYSSVV LATARDSGKK
     151  YAVKVLSKEY LIRQKKVKYV TVEKLALQKL NGTKGIFKLF FTFQDEASLY
     201  FLLEYAPHGD FLGLIKKYGS LNETCARYYA SQIIDAVDSL HNIGIIHRDI
     251  KPENILLDKN MKVKLTDFGT AKILPEEPSN TADGKPYFDL YAKSKSFVGT
     301  AEYVSPELLN DNYTDSRCDI WAFGCILYQM LAGKPPFKAA NEYLTFQKVM
     351  KIQYAFTAGF PQIVKDLVKK LLVRDPNDRL TIKQIKAHLF FHEVNFEDGS
     401  VWDDNPPEIQ PYKINAEAMK PLQKVSESDT TVKMANLQLA GNGHADTPLQ
     451  APAATSQEHS VISMTAATAA FNKDYTSQPK LGSKSSTSVR SASNNTDREV
     501  IQKKVSKNRA SVSSPSISTT SRGKDNRSRS SDAFWSRYLQ NMDERVLLMK
     551  EVALSTRNLE DSPVGLENVA LDYKNPLDIE PPTDSAGKFY KKMFLITNLG
     601  RALVFVKRRS LSMWEEQEFE LQFELELNDV EKIRFISDQV LEIDGSRTIF
     651  IGCKERAVLM KLWKLIHNGM TAKPKVVSPK SDHKMFDKFI LQKRQNTKKK
     701  NQAPPVPQSN RLINGLPDRC ILKTPEEGAL HTKRPTSLQT RSSSNYSKLL
     751  ARSTQMRKNM TRTDEK*

external links for Pkh1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Pkh1p
1) Casamayor A, et al.  (1999) Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr Biol 9(4):186-97
2) Sun Y, et al.  (2000) Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast. Mol Cell Biol 20(12):4411-9
3) Friant S, et al.  (2001) Sphingoid base signaling via Pkh kinases is required for endocytosis in yeast. EMBO J 20(23):6783-92
4) Roelants FM, et al.  (2002) Pkh1 and Pkh2 differentially phosphorylate and activate Ypk1 and Ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Mol Biol Cell 13(9):3005-28
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61