RMT2/YDR465C Protein Information

Standard Name	Rmt2p 1
Systematic Name	Ydr465cp
ORF Classification	Verified
Description	Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3)
Name Description	aRginine MeThyltransferase 1

Experimental Data
Molecules/cell	1600 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	412
Molecular Weight (Da)	47,469
Isoelectric Point (pI)	4.27

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rmt2p (InterPro)
Physical Interactions	There are 11 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006C5B6 \| Q03305 MIPS: YDR465C NCBI: 6320673 \| 74583411 \| 927761 \| NP_010753.1 GenBank/EMBL/DDBJ: DAA12300.1 \| U33050
External Classifications	EC: 2.1.1.- [Transferases transferring one-carbon groups methyltransferases]

Amino Acid Sequence (or in FASTA format)
1 MSELHALLTF PERPISQSYY VPKLQHFLKS GIPATYTLEQ VAAFEHEEKN 51 RNGDKEFRES TDDNKTSNTT PLHVLARSLP LDIKDEELQV VMDMMNILFE 101 YGAGWNFIDY EDKTVGDLFL ERNQSRESPL YRRLVEAGVS AELLLRKLNG 151 GDVEFLDTDE LIGIEPEESV QTAVDGQKEE SVGSDDDATA ANQQVYLKTE 201 LEYKDDALIT KENKDGVMMD WETKIMELAS ETLFPDPEAT NSATILNIGF 251 GMGIIDTFIQ ARKPYRHYIC EAHPDVLAKM KMDGWYEKDN VVILEGRWQD 301 TLNNLLDKGE VFFDGIYYDT FSEHYQDILD LYDVVVGLIK PEGVFSFFNG 351 LGADRSLCYD VYKEIVEIDV ATYGMKCDYT RYSLDEQLPD WNDVKRSYFN 401 CNYYYHPRIT FA*

Amino Acid Sequence (or in FASTA format)

       1  MSELHALLTF PERPISQSYY VPKLQHFLKS GIPATYTLEQ VAAFEHEEKN
      51  RNGDKEFRES TDDNKTSNTT PLHVLARSLP LDIKDEELQV VMDMMNILFE
     101  YGAGWNFIDY EDKTVGDLFL ERNQSRESPL YRRLVEAGVS AELLLRKLNG
     151  GDVEFLDTDE LIGIEPEESV QTAVDGQKEE SVGSDDDATA ANQQVYLKTE
     201  LEYKDDALIT KENKDGVMMD WETKIMELAS ETLFPDPEAT NSATILNIGF
     251  GMGIIDTFIQ ARKPYRHYIC EAHPDVLAKM KMDGWYEKDN VVILEGRWQD
     301  TLNNLLDKGE VFFDGIYYDT FSEHYQDILD LYDVVVGLIK PEGVFSFFNG
     351  LGADRSLCYD VYKEIVEIDV ATYGMKCDYT RYSLDEQLPD WNDVKRSYFN
     401  CNYYYHPRIT FA*

external links for Rmt2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Rmt2p

1)	Niewmierzycka A and Clarke S (1999) S-Adenosylmethionine-dependent methylation in Saccharomyces cerevisiae. Identification of a novel protein arginine methyltransferase. J Biol Chem 274(2):814-24
2)	Chern MK, et al. (2002) Yeast ribosomal protein L12 is a substrate of protein-arginine methyltransferase 2. J Biol Chem 277(18):15345-53
3)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41