GUK1/YDR454C Protein Information

Standard Name	Guk1p
Systematic Name	Ydr454cp
Alias	Pur5p
ORF Classification	Verified
Description	Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins (1, 2, 3)
Name Description	GUanylate Kinase 1

Experimental Data
Molecules/cell	20500 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	187
Molecular Weight (Da)	20,637
Isoelectric Point (pI)	7.31

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Guk1p (InterPro)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000168305 \| P15454 MIPS: YDR454C NCBI: 13787040 \| 13787041 \| 13787042 \| 171625 \| 334878447 \| 390981224 \| 390981225 \| 417211 \| 6320662 \| 927715 \| NP_010742.1 GenBank/EMBL/DDBJ: DAA12289.1 \| L04683 \| U33007
External Classifications	EC: 2.7.4.8 [Guanylate kinase]

Amino Acid Sequence (or in FASTA format)
1 MSRPIVISGP SGTGKSTLLK KLFAEYPDSF GFSVSSTTRT PRAGEVNGKD 51 YNFVSVDEFK SMIKNNEFIE WAQFSGNYYG STVASVKQVS KSGKTCILDI 101 DMQGVKSVKA IPELNARFLF IAPPSVEDLK KRLEGRGTET EESINKRLSA 151 AQAELAYAET GAHDKVIVND DLDKAYKELK DFIFAEK*

Amino Acid Sequence (or in FASTA format)

       1  MSRPIVISGP SGTGKSTLLK KLFAEYPDSF GFSVSSTTRT PRAGEVNGKD
      51  YNFVSVDEFK SMIKNNEFIE WAQFSGNYYG STVASVKQVS KSGKTCILDI
     101  DMQGVKSVKA IPELNARFLF IAPPSVEDLK KRLEGRGTET EESINKRLSA
     151  AQAELAYAET GAHDKVIVND DLDKAYKELK DFIFAEK*

external links for Guk1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Guk1p

1)	Konrad M (1992) Cloning and expression of the essential gene for guanylate kinase from yeast. J Biol Chem 267(36):25652-5
2)	Shimma Y, et al. (1997) A defect in GTP synthesis affects mannose outer chain elongation in Saccharomyces cerevisiae. Mol Gen Genet 256(5):469-80
3)	Berger A, et al. (1989) Guanylate kinase from Saccharomyces cerevisiae. Isolation and characterization, crystallization and preliminary X-ray analysis, amino acid sequence and comparison with adenylate kinases. Eur J Biochem 184(2):433-43
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41