TSA2/YDR453C Protein Information

Standard Name	Tsa2p
Systematic Name	Ydr453cp
ORF Classification	Verified
Description	Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description	Thiol-Specific Antioxidant
Gene Product	cTPxII 1

Experimental Data
Molecules/cell	4820 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	196
Molecular Weight (Da)	21,615
Isoelectric Point (pI)	7.34

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tsa2p (InterPro)
Physical Interactions	There are 18 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000168304 \| Q04120 MIPS: YDR453C NCBI: 2499475 \| 6320661 \| 927720 \| NP_010741.1 GenBank/EMBL/DDBJ: DAA12288.1 \| U33007
External Classifications	EC: 1.11.1.15 [Peroxiredoxin]

Amino Acid Sequence (or in FASTA format)
1 MVAEVQKQAP PFKKTAVVDG IFEEISLEKY KGKYVVLAFV PLAFSFVCPT 51 EIVAFSDAAK KFEDQGAQVL FASTDSEYSL LAWTNLPRKD GGLGPVKVPL 101 LADKNHSLSR DYGVLIEKEG IALRGLFIID PKGIIRHITI NDLSVGRNVN 151 EALRLVEGFQ WTDKNGTVLP CNWTPGAATI KPDVKDSKEY FKNANN*

Amino Acid Sequence (or in FASTA format)

       1  MVAEVQKQAP PFKKTAVVDG IFEEISLEKY KGKYVVLAFV PLAFSFVCPT
      51  EIVAFSDAAK KFEDQGAQVL FASTDSEYSL LAWTNLPRKD GGLGPVKVPL
     101  LADKNHSLSR DYGVLIEKEG IALRGLFIID PKGIIRHITI NDLSVGRNVN
     151  EALRLVEGFQ WTDKNGTVLP CNWTPGAATI KPDVKDSKEY FKNANN*

external links for Tsa2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Tsa2p

1)	Park SG, et al. (2000) Distinct physiological functions of thiol peroxidase isoenzymes in Saccharomyces cerevisiae. J Biol Chem 275(8):5723-32
2)	Wong CM, et al. (2002) Cooperation of yeast peroxiredoxins Tsa1p and Tsa2p in the cellular defense against oxidative and nitrosative stress. J Biol Chem 277(7):5385-94
3)	Wong CM, et al. (2004) Peroxiredoxin-null yeast cells are hypersensitive to oxidative stress and are genomically unstable. J Biol Chem 279(22):23207-13
4)	Munhoz DC and Netto LE (2004) Cytosolic thioredoxin peroxidase I and II are important defenses of yeast against organic hydroperoxide insult: catalases and peroxiredoxins cooperate in the decomposition of H2O2 by yeast. J Biol Chem 279(34):35219-27
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41