SSN2/YDR443C Protein Information

Standard Name	Ssn2p 1
Systematic Name	Ydr443cp
Alias	Nut8p 2 , Sca1p , Srb9p , Ume2p , Rye3p , Med13p 3
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation (1, 4, 5)
Name Description	Suppressor of SNf1 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,420
Molecular Weight (Da)	159,999
Isoelectric Point (pI)	5.57

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ssn2p (InterPro)
Physical Interactions	There are 69 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053161 \| P38931 MIPS: YDR443C NCBI: 1351114 \| 398366601 \| 495490 \| 780741 \| 927709 \| NP_010731.3 GenBank/EMBL/DDBJ: DAA12279.1 \| U09176 \| U23812 \| U33007

Amino Acid Sequence (or in FASTA format)
1 MSSDASTYRL EDVLSSFYRV EKIKKINYHQ YISKAQNDQW SIQMEFMLRK 51 QDPKTLVALL SRDLWCFSIN DDPVPTPPAI EHKPVSPDKI GTFTADYSKP 101 NLPPHYALFL KALRRKIYIN LALGSHNKLI QFGNACISLS GVPNYLVQLE 151 PHLFVNGDLT VSLCAKNMGL VPMKEENLEE SFLSKHALYL APSGIRMHLA 201 PASKQGYLIT PPKHTELLLT TLSVSHGINL QNKKNLKWVA VVPDLGHLNG 251 HTPTIASYLT PLLEAKKLVW PLHLIFAQPV ADIENSTSGD PSEFHCLQDA 301 LDAIDDFIQL KQTAAYRTPG SSGVLSSNIA GTNPLSSDGA YTEQFQHYKN 351 NSISSQPASY HSVQETNKIS PKDFSPNFTG IDKLMLSPSD QFAPAFLNTP 401 NNNINENELF NDRKQTTVSN DLENSPLKTE LEANGRSLEK VNNSVSKTGS 451 VDTLHNKEGT LEQREQNENL PSDKSDSMVD KELFGEDEDE DLFGDSNKSN 501 STNESNKSIS DEITEDMFEM SDEEENNNNK SINKNNKEMH TDLGKDIPFF 551 PSSEKPNIRT MSGTTKRLNG KRKYLDIPID EMTLPTSPLY MDPGAPLPVE 601 TPRDRRKSVF APLNFNPIIE NNVDNKYKSG GKFSFSPLQK EEALNFDISM 651 ADLSSSEEEE DEEENGSSDE DLKSLNVRDD MKPSDNISTN TNIHEPQYIN 701 YSSIPSLQDS IIKQENFNSV NDANITSNKE GFNSIWKIPQ NDIPQTESPL 751 KTVDSSIQPI ESNIKMTLED NNVTSNPSEF TPNMVNSEIS NLPKDKSGIP 801 EFTPADPNLS FESSSSLPFL LRHMPLASIP DIFITPTPVV TISEKEQDIL 851 DLIAEQVVTD YNILGNLGIP KIAYRGVKDC QEGLITTTML QLFSTFDRLN 901 GNDTISKFYN MKQPYVFVKK HHELIKVKHD SQPFIKFLNF RPPNGIKNFK 951 SLLLSSSFKE DCLSFAPTLS QTYINQELGF CELLKLTNED PPGLMYLKAF 1001 DKNKLLLLAA QIVSYCSNNK NSIKNVPPIL IILPLDNATL TELVDKANIF 1051 QVIKNEVCAK MPNIELYLKV IPMDFIRNVL VTVDQYVNVA ISIYNMLPPK 1101 SVKFTHIAHT LPEKVNFRTM QQQQMQQQQQ QQQQQQNNST GSSSIIYYDS 1151 YIHLAYSRSV DKEWVFAALS DSYGQGSMTK TWYVGNSRGK FDDACNQIWN 1201 IALNLASKKF GKICLILTRL NGILPDDELM NWRRLSGRNI HLAVVCVDDN 1251 SKISFIDEDK LYPSFKPIYK DTRFGGRMDM TRLYDYEIRD IDQDIHGIVF 1301 QHPFPLAHSQ HRCAIRSGAL IKFKKCDGDT VWDKFAVNLL NCPHSDSTQL 1351 LETILEEFRN LAALNVWYGL SDGEDGHIPW HILAVKKMMN TLVHTRVKIA 1401 NTSAATVHTA TSSSIILSDK *

Amino Acid Sequence (or in FASTA format)

       1  MSSDASTYRL EDVLSSFYRV EKIKKINYHQ YISKAQNDQW SIQMEFMLRK
      51  QDPKTLVALL SRDLWCFSIN DDPVPTPPAI EHKPVSPDKI GTFTADYSKP
     101  NLPPHYALFL KALRRKIYIN LALGSHNKLI QFGNACISLS GVPNYLVQLE
     151  PHLFVNGDLT VSLCAKNMGL VPMKEENLEE SFLSKHALYL APSGIRMHLA
     201  PASKQGYLIT PPKHTELLLT TLSVSHGINL QNKKNLKWVA VVPDLGHLNG
     251  HTPTIASYLT PLLEAKKLVW PLHLIFAQPV ADIENSTSGD PSEFHCLQDA
     301  LDAIDDFIQL KQTAAYRTPG SSGVLSSNIA GTNPLSSDGA YTEQFQHYKN
     351  NSISSQPASY HSVQETNKIS PKDFSPNFTG IDKLMLSPSD QFAPAFLNTP
     401  NNNINENELF NDRKQTTVSN DLENSPLKTE LEANGRSLEK VNNSVSKTGS
     451  VDTLHNKEGT LEQREQNENL PSDKSDSMVD KELFGEDEDE DLFGDSNKSN
     501  STNESNKSIS DEITEDMFEM SDEEENNNNK SINKNNKEMH TDLGKDIPFF
     551  PSSEKPNIRT MSGTTKRLNG KRKYLDIPID EMTLPTSPLY MDPGAPLPVE
     601  TPRDRRKSVF APLNFNPIIE NNVDNKYKSG GKFSFSPLQK EEALNFDISM
     651  ADLSSSEEEE DEEENGSSDE DLKSLNVRDD MKPSDNISTN TNIHEPQYIN
     701  YSSIPSLQDS IIKQENFNSV NDANITSNKE GFNSIWKIPQ NDIPQTESPL
     751  KTVDSSIQPI ESNIKMTLED NNVTSNPSEF TPNMVNSEIS NLPKDKSGIP
     801  EFTPADPNLS FESSSSLPFL LRHMPLASIP DIFITPTPVV TISEKEQDIL
     851  DLIAEQVVTD YNILGNLGIP KIAYRGVKDC QEGLITTTML QLFSTFDRLN
     901  GNDTISKFYN MKQPYVFVKK HHELIKVKHD SQPFIKFLNF RPPNGIKNFK
     951  SLLLSSSFKE DCLSFAPTLS QTYINQELGF CELLKLTNED PPGLMYLKAF
    1001  DKNKLLLLAA QIVSYCSNNK NSIKNVPPIL IILPLDNATL TELVDKANIF
    1051  QVIKNEVCAK MPNIELYLKV IPMDFIRNVL VTVDQYVNVA ISIYNMLPPK
    1101  SVKFTHIAHT LPEKVNFRTM QQQQMQQQQQ QQQQQQNNST GSSSIIYYDS
    1151  YIHLAYSRSV DKEWVFAALS DSYGQGSMTK TWYVGNSRGK FDDACNQIWN
    1201  IALNLASKKF GKICLILTRL NGILPDDELM NWRRLSGRNI HLAVVCVDDN
    1251  SKISFIDEDK LYPSFKPIYK DTRFGGRMDM TRLYDYEIRD IDQDIHGIVF
    1301  QHPFPLAHSQ HRCAIRSGAL IKFKKCDGDT VWDKFAVNLL NCPHSDSTQL
    1351  LETILEEFRN LAALNVWYGL SDGEDGHIPW HILAVKKMMN TLVHTRVKIA
    1401  NTSAATVHTA TSSSIILSDK *

external links for Ssn2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ssn2p

1)	Carlson M, et al. (1984) A suppressor of SNF1 mutations causes constitutive high-level invertase synthesis in yeast. Genetics 107(1):19-32
2)	Tabtiang RK and Herskowitz I (1998) Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol 18(8):4707-18
3)	Bourbon HM, et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
4)	Song W, et al. (1996) SSN genes that affect transcriptional repression in Saccharomyces cerevisiae encode SIN4, ROX3, and SRB proteins associated with RNA polymerase II. Mol Cell Biol 16(1):115-20
5)	Larschan E and Winston F (2005) The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription. Mol Cell Biol 25(1):114-23