DOT1/YDR440W Protein Information Help

Standard Name Dot1p 1
Systematic Name Ydr440wp
Alias Pch1p 2
ORF Classification Verified
Description Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response (2, 3, 4, 5)
Name Description Disruptor Of Telomeric silencing 1
Gene Product KMT4 6
Experimental Data
Molecules/cell 2160 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 582
Molecular Weight (Da) 66,201
Isoelectric Point (pI) 9.76

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dot1p (InterPro)
Physical Interactions There are 32 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001296B4 | Q04089
MIPS: YDR440W
NCBI: 21759096 | 55670156 | 55670157 | 55670158 | 6320648 | 927702 | NP_010728.1
GenBank/EMBL/DDBJ: DAA12277.1 | U33007
External Classifications EC: 2.1.1.43 [Histone-lysine N-methyltransferase]
Amino Acid Sequence (or in FASTA format)
 
       1  MGGQESISNN NSDSFIMSSP NLDSQESSIS PIDEKKGTDM QTKSLSSYSK
      51  GTLLSKQVQN LLEEANKYDP IYGSSLPRGF LRDRNTKGKD NGLVPLVEKV
     101  IPPIHKKTNN RNTRKKSSTT TKKDVKKPKA AKVKGKNGRT NHKHTPISKQ
     151  EIDTAREKKP LKKGRANKKN DRDSPSSTFV DWNGPCLRLQ YPLFDIEYLR
     201  SHEIYSGTPI QSISLRTNSP QPTSLTSDND TSSVTTAKLQ SILFSNYMEE
     251  YKVDFKRSTA IYNPMSEIGK LIEYSCLVFL PSPYAEQLKE TILPDLNASF
     301  DNSDTKGFVN AINLYNKMIR EIPRQRIIDH LETIDKIPRS FIHDFLHIVY
     351  TRSIHPQANK LKHYKAFSNY VYGELLPNFL SDVYQQCQLK KGDTFMDLGS
     401  GVGNCVVQAA LECGCALSFG CEIMDDASDL TILQYEELKK RCKLYGMRLN
     451  NVEFSLKKSF VDNNRVAELI PQCDVILVNN FLFDEDLNKK VEKILQTAKV
     501  GCKIISLKSL RSLTYQINFY NVENIFNRLK VQRYDLKEDS VSWTHSGGEY
     551  YISTVMEDVD ESLFSPAARG RRNRGTPVKY TR*

external links for Dot1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
YGOB CYC2008 (complexes) Pfam domains
YOGY Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Dot1p
1) Singer MS, et al.  (1998) Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae. Genetics 150(2):613-32
2) San-Segundo PA and Roeder GS  (2000) Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell 11(10):3601-15
3) van Leeuwen F, et al.  (2002) Dot1p modulates silencing in yeast by methylation of the nucleosome core. Cell 109(6):745-56
4) Game JC, et al.  (2005) X-ray survival characteristics and genetic analysis for nine Saccharomyces deletion mutants that show altered radiation sensitivity. Genetics 169(1):51-63
5) Wysocki R, et al.  (2005) Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9. Mol Cell Biol 25(19):8430-43
6) Allis CD, et al.  (2007) New nomenclature for chromatin-modifying enzymes. Cell 131(4):633-6
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41