UBA2/YDR390C Protein Information

Standard Name	Uba2p
Systematic Name	Ydr390cp
Alias	Ual1p
ORF Classification	Verified
Description	Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability (1, 2)
Name Description	UBiquitin Activating 1

Experimental Data
Molecules/cell	18800 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	636
Molecular Weight (Da)	71,258
Isoelectric Point (pI)	4.83

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Uba2p (InterPro)
Physical Interactions	There are 26 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005325E \| P52488 MIPS: YDR390C NCBI: 1717852 \| 1835684 \| 317455354 \| 317455356 \| 317455358 \| 398366555 \| 475916 \| 793879 \| 927323 \| NP_010678.3 GenBank/EMBL/DDBJ: DAA12234.1 \| U17263 \| U32274 \| Z30326 \| Z48725

Amino Acid Sequence (or in FASTA format)
1 MPRETSLVTI IGEDSYKKLR SSRCLLVGAG GIGSELLKDI ILMEFGEIHI 51 VDLDTIDLSN LNRQFLFRQK DIKQPKSTTA VKAVQHFNNS KLVPYQGNVM 101 DISTFPLHWF EQFDIIFNAL DNLAARRYVN KISQFLSLPL IESGTAGFDG 151 YMQPIIPGKT ECFECTKKET PKTFPVCTIR STPSQPIHCI VWAKNFLFNQ 201 LFASETSGNE DDNNQDWGTD DAEEIKRIKQ ETNELYELQK IIISRDASRI 251 PEILNKLFIQ DINKLLAIEN LWKTRTKPVP LSDSQINTPT KTAQSASNSV 301 GTIQEQISNF INITQKLMDR YPKEQNHIEF DKDDADTLEF VATAANIRSH 351 IFNIPMKSVF DIKQIAGNII PAIATTNAIV AGASSLISLR VLNLLKYAPT 401 TKYTDLNMAF TAKASNLSQN RYLSNPKLAP PNKNCPVCSK VCRGVIKLSS 451 DCLNKMKLSD FVVLIREKYS YPQDISLLDA SNQRLLFDYD FEDLNDRTLS 501 EINLGNGSII LFSDEEGDTM IRKAIELFLD VDDELPCNTC SLPDVEVPLI 551 KANNSPSKNE EEEKNEKGAD VVATTNSHGK DGIVILDDDE GEITIDAEPI 601 NGSKKRPVDT EISEAPSNKR TKLVNEPTNS DIVELD*

Amino Acid Sequence (or in FASTA format)

       1  MPRETSLVTI IGEDSYKKLR SSRCLLVGAG GIGSELLKDI ILMEFGEIHI
      51  VDLDTIDLSN LNRQFLFRQK DIKQPKSTTA VKAVQHFNNS KLVPYQGNVM
     101  DISTFPLHWF EQFDIIFNAL DNLAARRYVN KISQFLSLPL IESGTAGFDG
     151  YMQPIIPGKT ECFECTKKET PKTFPVCTIR STPSQPIHCI VWAKNFLFNQ
     201  LFASETSGNE DDNNQDWGTD DAEEIKRIKQ ETNELYELQK IIISRDASRI
     251  PEILNKLFIQ DINKLLAIEN LWKTRTKPVP LSDSQINTPT KTAQSASNSV
     301  GTIQEQISNF INITQKLMDR YPKEQNHIEF DKDDADTLEF VATAANIRSH
     351  IFNIPMKSVF DIKQIAGNII PAIATTNAIV AGASSLISLR VLNLLKYAPT
     401  TKYTDLNMAF TAKASNLSQN RYLSNPKLAP PNKNCPVCSK VCRGVIKLSS
     451  DCLNKMKLSD FVVLIREKYS YPQDISLLDA SNQRLLFDYD FEDLNDRTLS
     501  EINLGNGSII LFSDEEGDTM IRKAIELFLD VDDELPCNTC SLPDVEVPLI
     551  KANNSPSKNE EEEKNEKGAD VVATTNSHGK DGIVILDDDE GEITIDAEPI
     601  NGSKKRPVDT EISEAPSNKR TKLVNEPTNS DIVELD*

external links for Uba2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Uba2p

1)	Dohmen RJ, et al. (1995) An essential yeast gene encoding a homolog of ubiquitin-activating enzyme. J Biol Chem 270(30):18099-109
2)	Johnson ES, et al. (1997) The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer. EMBO J 16(18):5509-19
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41