SAC7/YDR389W Protein Information Help

Standard Name Sac7p 1
Systematic Name Ydr389wp
ORF Classification Verified
Description GTPase activating protein (GAP) for Rho1p; involved in signaling to actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Suppressor of ACtin 6
Experimental Data
Molecules/cell 1420 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 654
Molecular Weight (Da) 73,629
Isoelectric Point (pI) 10.4

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sac7p (InterPro)
Physical Interactions There are 11 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001354D1 | P17121
MIPS: YDR389W
NCBI: 172532 | 28381398 | 6320597 | 927322 | NP_010677.1
GenBank/EMBL/DDBJ: DAA12233.1 | M32335 | U32274
Amino Acid Sequence (or in FASTA format)
 
       1  MPNNTLKQGS KIENVSPSKG HVPSFWKQFI NNPKSMSSEN ITVPRSPTSL
      51  SRNAQPTTLK RPPLSSRPYS YNTPTKDRKS FSKSAKQNNN NNNANSGTSP
     101  HAEFKNYRDM FLSNRNGFTG RVFGVTLAES LSVASAEVIV QSELVSFGRI
     151  PIVVAKCGAY LKANGLETSG IFRIAGNGKR VKALQYIFSS PPDYGTKFND
     201  WETYTVHDVA SLLRRYLNNL AEPLIPLSLY EQFRNPLRSR PRILRHMLTH
     251  EVSHPNANKT NNVTVKSSRQ NYNDDGANDG DIEKEDAKDD EEKRRRKIRH
     301  KRRLTRDIRA AIKEYEELFV TLSNDTKQLT IYLLDLLSLF ARQSQFNLMS
     351  GRNLAAIFQP SILSHPQHDM DPKEYELSRL VVEFLIEYSY KLLPHLLKLA
     401  KREQQERLST ENKKNNGDKQ KTDPIEIPKI TSSDSPPIVS SNKNPPAIDN
     451  NNKLDHTTLS PISTSIPENS SDLQTSKMLK PPKQRRPHSK SFGSTPVPPD
     501  VIASNKRRTS LFPWLHKPGI LSDTGDNGDL TATEAEGDDY EEENVDPYGQ
     551  SPSSVHSGSL PKQHYLPIPR MNRSLSGNST NSSFNTRPIS MILTSGNDNS
     601  ADQLELLSNT HSNNERSNAL PLTEDDGDER NSRSRKRESW FQRLTSRSGS
     651  ANRA*

external links for Sac7p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains YeastRC Public Image Repository

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Sac7p
1) Dunn TM and Shortle D  (1990) Null alleles of SAC7 suppress temperature-sensitive actin mutations in Saccharomyces cerevisiae. Mol Cell Biol 10(5):2308-14
2) Schmidt A, et al.  (1997) The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2. Cell 88(4):531-42
3) Schmidt A, et al.  (2002) The RHO1-GAPs SAC7, BEM2 and BAG7 control distinct RHO1 functions in Saccharomyces cerevisiae. Mol Microbiol 45(5):1433-41
4) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Novick P, et al.  (1989) Suppressors of yeast actin mutations. Genetics 121(4):659-74
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41