MUS81/YDR386W Protein Information Help

Standard Name Mus81p 1
Systematic Name Ydr386wp
Alias Slx3p
ORF Classification Verified
Description Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p (1, 2, 3, 4, 5, 6)
Name Description MMS and UV Sensitive 1
Experimental Data
Molecules/cell 300 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 632
Molecular Weight (Da) 72,308
Isoelectric Point (pI) 8.61

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mus81p (InterPro)
Physical Interactions There are 50 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006A8D4 | Q04149
MIPS: YDR386W
NCBI: 398366551 | 46396280 | 927319 | NP_010674.3
GenBank/EMBL/DDBJ: DAA12230.1 | U32274
External Classifications EC: 3.1.22.- [Endodeoxyribonucleases Producing 3'-Phosphomonoesters]
Amino Acid Sequence (or in FASTA format)
 
       1  MELSSNLKDL YIEWLQELVD GLTPKQEQLK IAYEKAKRNL QNAEGSFYYP
      51  TDLKKVKGIG NTIIKRLDTK LRNYCKIHHI SPVEAPSLTQ TSSTRPPKRT
     101  TTALRSIVNS CENDKNEAPE EKGTKKRKTR KYIPKKRSGG YAILLSLLEL
     151  NAIPRGVSKE QIIEVAGKYS DHCMTPNFST KEFYGAWSSI AALKKHSLVL
     201  EEGRPKRYSL TEEGVELTKS LKTADGISFP KENEEPNEYS VTRNESSEFT
     251  ANLTDLRGEY GKEEEPCDIN NTSFMLDITF QDLSTPQRLQ NNVFKNDRLN
     301  SQTNISSHKL EEVSDDQTVP DSALKAKSTI KRRRYNGVSY ELWCSGDFEV
     351  FPIIDHREIK SQSDREFFSR AFERKGMKSE IRQLALGDII WVAKNKNTGL
     401  QCVLNTIVER KRLDDLALSI RDNRFMEQKN RLEKSGCEHK YYLIEETMSG
     451  NIGNMNEALK TALWVILVYY KFSMIRTCNS DETVEKIHAL HTVISHHYSQ
     501  KDLIVIFPSD LKSKDDYKKV LLQFRREFER KGGIECCHNL ECFQELMGKG
     551  DLKTVGELTI HVLMLVKGIS LEKAVAIQEI FPTLNKILMA YKTCSSEEEA
     601  KLLMFNVLGD APGAKKITKS LSEKIYDAFG KL*

external links for Mus81p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mus81p
1) Interthal H and Heyer WD  (2000) MUS81 encodes a novel helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae. Mol Gen Genet 263(5):812-27
2) Bastin-Shanower SA, et al.  (2003) The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10. Mol Cell Biol 23(10):3487-96
3) Fu Y and Xiao W  (2003) Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization. DNA Repair (Amst) 2(12):1435-47
4) Oh SD, et al.  (2008) RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell 31(3):324-36
5) Jessop L and Lichten M  (2008) Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell 31(3):313-23
6) Gallo-Fernandez M, et al.  (2012) Cell cycle-dependent regulation of the nuclease activity of Mus81-Eme1/Mms4. Nucleic Acids Res 40(17):8325-35
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41