BCS1/YDR375C Protein Information

Standard Name	Bcs1p
Systematic Name	Ydr375cp
ORF Classification	Verified
Description	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases (1, 2, 3)
Name Description	ubiquinol-cytochrome c reductase (bc1) Synthesis

Experimental Data
Molecules/cell	1990 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	456
Molecular Weight (Da)	51,107
Isoelectric Point (pI)	9.5

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bcs1p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000126849 \| P32839 MIPS: YDR375C NCBI: 2506091 \| 258018 \| 6320583 \| 849196 \| NP_010663.1 GenBank/EMBL/DDBJ: DAA12217.1 \| S47190 \| U28373

Amino Acid Sequence (or in FASTA format)
1 MSDKPIDIQY DKQATPNLSG VITPPTNETG NDSVREKLSK LVGDAMSNNP 51 YFAAGGGLMI LGTGLAVARS GIIKASRVLY RQMIVDLEIQ SKDKSYAWFL 101 TWMAKHPQRV SRHLSVRTNY IQHDNGSVST KFSLVPGPGN HWIRYKGAFI 151 LIKRERSAKM IDIANGSPFE TVTLTTLYRD KHLFDDILNE AKDIALKTTE 201 GKTVIYTSFG PEWRKFGQPK AKRMLPSVIL DSGIKEGILD DVYDFMKNGK 251 WYSDRGIPYR RGYLLYGPPG SGKTSFIQAL AGELDYNICI LNLSENNLTD 301 DRLNHLMNNM PERSILLLED IDAAFNKRSQ TGEQGFHSSV TFSGLLNALD 351 GVTSSEETIT FMTTNHPEKL DAAIMRPGRI DYKVFVGNAT PYQVEKMFMK 401 FYPGETDICK KFVNSVKELD ITVSTAQLQG LFVMNKDAPH DALKMVSSLR 451 NANHIF*

Amino Acid Sequence (or in FASTA format)

       1  MSDKPIDIQY DKQATPNLSG VITPPTNETG NDSVREKLSK LVGDAMSNNP
      51  YFAAGGGLMI LGTGLAVARS GIIKASRVLY RQMIVDLEIQ SKDKSYAWFL
     101  TWMAKHPQRV SRHLSVRTNY IQHDNGSVST KFSLVPGPGN HWIRYKGAFI
     151  LIKRERSAKM IDIANGSPFE TVTLTTLYRD KHLFDDILNE AKDIALKTTE
     201  GKTVIYTSFG PEWRKFGQPK AKRMLPSVIL DSGIKEGILD DVYDFMKNGK
     251  WYSDRGIPYR RGYLLYGPPG SGKTSFIQAL AGELDYNICI LNLSENNLTD
     301  DRLNHLMNNM PERSILLLED IDAAFNKRSQ TGEQGFHSSV TFSGLLNALD
     351  GVTSSEETIT FMTTNHPEKL DAAIMRPGRI DYKVFVGNAT PYQVEKMFMK
     401  FYPGETDICK KFVNSVKELD ITVSTAQLQG LFVMNKDAPH DALKMVSSLR
     451  NANHIF*

external links for Bcs1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Bcs1p

1)	Cruciat CM, et al. (1999) Bcs1p, an AAA-family member, is a chaperone for the assembly of the cytochrome bc(1) complex. EMBO J 18(19):5226-33
2)	Tuppen HA, et al. (2010) Long-term survival of neonatal mitochondrial complex III deficiency associated with a novel BCS1L gene mutation. Mol Genet Metab 100(4):345-8
3)	Wagener N, et al. (2011) A pathway of protein translocation in mitochondria mediated by the AAA-ATPase Bcs1. Mol Cell 44(2):191-202
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41