MSN5/YDR335W Protein Information

Standard Name	Msn5p
Systematic Name	Ydr335wp
Alias	Kap142p , Ste21p 1
ORF Classification	Verified
Description	Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm (2, 3, 4, 5, 6, 7)
Name Description	Multicopy suppressor of SNF1 mutation

Experimental Data
Molecules/cell	3500 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,224
Molecular Weight (Da)	142,116
Isoelectric Point (pI)	5.07

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Msn5p (InterPro)
Physical Interactions	There are 73 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012F625 \| P52918 MIPS: YDR335W NCBI: 1230662 \| 1246837 \| 1709133 \| 6320542 \| NP_010622.1 GenBank/EMBL/DDBJ: DAA12177.1 \| U51032 \| X93302

Amino Acid Sequence (or in FASTA format)
1 MDSTGASQIV SALDVIYSPK SNNSQRQEAQ KFLDEVKLCS ESPFWGYEIA 51 LQNPTNSILK YFGLGLLDHA VKKNWNDYDE GKRVALRKWV MELNFGVQDY 101 DTRYIKEKLA TLWVEVAKRT WGEALKQTNP TEEQLLTSWV DMDNNLFELW 151 NINQSSRELA LIIFRILFED VFLLDDLIVL KRMTVIQPLC VMIVCPIEVF 201 AIKYKFSDKW TKFKANEEGW FSVWIPELNN ALQQNNSEYI IRLLETLKTC 251 LNWPLTEVIV RNDVLSSLLT CLSSNIPRAQ SMALDSIHIL LTRPYSNESH 301 YQMTIDRVFD NMDLLDSVYE SLLFDPTDDI DETKYPIIKK FVDMISCLYV 351 CVPKIKETNG QIQKYFKLVL KTTYNPSLIV SGLTLDLWCT CLRNDEYLPK 401 LEKYVIPDLL QFAADALVYY EQIDGHISKK FAEIDFQSKS EFQTFCSTYR 451 KRIRDIIRLI SCVELDLTYD WLNNRLNNYF SSPFGQQVLS STFLDHKLEP 501 YLGALSQYMI VECFINGCIR WKIWYPTGDD YDEKLDSILQ KLEILSNQLI 551 ALNLREPLLL KKQIQNFALF LTMLKDNVLF TLLEKIITSA TMDYPEINLE 601 ERGAESDAVR DLRYACGIEL NRMALLMPES LKKIYPDLES VIARIMPNLS 651 YHEKISFKSF LLIIVLKSSL DMKEERFAAI VDPELLAWSD KTTVVGLSDL 701 HWFMERLGIV QIAEYFQRRD IDENSDLLSI PIDDEGKELK SELTKRWQSL 751 FPVRATRMFI HYSMQSIKTD EEFKMLQDLW RPRIVPILPY ITRLLYQLQS 801 YHDPDNWKGL PTVVQSFVKY STIERFWEAG ASNKSKDEFI DEHMKAMQTL 851 RDFADSVGHI IRYTREYTLL VLSAISSLGS VFYLLDESPD LLLNSIAIFK 901 PGSNEISPGV STHGWKHIMN IAIRPILKGC PKDCLGKFMP AFLPKLFEIL 951 DLLLCQKWSS HMNDMDMNPV PTDDDQMTEE ILEENLLRQL TTVVVRIVID 1001 CVGQGNANPN SAKSRLNNHQ MEMRKIIFND LNTLAPFLKL LNHLISFKDT 1051 KCSFNSILVM KCCLTSVLNQ NNTVDEYFTF EVMKNLLLNV LCNSAFKDSF 1101 HEALYAFTVI FLTLCKEYPS ARAFLFEISN GYNIDELYRN LRSVDEYKTQ 1151 RALMIDFIDW VKSTSGKEDG NVDHAGDERK RQEKREAILK KANERLIKKN 1201 KENGDMLDDP NIEDGAVGNL FDDN*

Amino Acid Sequence (or in FASTA format)

       1  MDSTGASQIV SALDVIYSPK SNNSQRQEAQ KFLDEVKLCS ESPFWGYEIA
      51  LQNPTNSILK YFGLGLLDHA VKKNWNDYDE GKRVALRKWV MELNFGVQDY
     101  DTRYIKEKLA TLWVEVAKRT WGEALKQTNP TEEQLLTSWV DMDNNLFELW
     151  NINQSSRELA LIIFRILFED VFLLDDLIVL KRMTVIQPLC VMIVCPIEVF
     201  AIKYKFSDKW TKFKANEEGW FSVWIPELNN ALQQNNSEYI IRLLETLKTC
     251  LNWPLTEVIV RNDVLSSLLT CLSSNIPRAQ SMALDSIHIL LTRPYSNESH
     301  YQMTIDRVFD NMDLLDSVYE SLLFDPTDDI DETKYPIIKK FVDMISCLYV
     351  CVPKIKETNG QIQKYFKLVL KTTYNPSLIV SGLTLDLWCT CLRNDEYLPK
     401  LEKYVIPDLL QFAADALVYY EQIDGHISKK FAEIDFQSKS EFQTFCSTYR
     451  KRIRDIIRLI SCVELDLTYD WLNNRLNNYF SSPFGQQVLS STFLDHKLEP
     501  YLGALSQYMI VECFINGCIR WKIWYPTGDD YDEKLDSILQ KLEILSNQLI
     551  ALNLREPLLL KKQIQNFALF LTMLKDNVLF TLLEKIITSA TMDYPEINLE
     601  ERGAESDAVR DLRYACGIEL NRMALLMPES LKKIYPDLES VIARIMPNLS
     651  YHEKISFKSF LLIIVLKSSL DMKEERFAAI VDPELLAWSD KTTVVGLSDL
     701  HWFMERLGIV QIAEYFQRRD IDENSDLLSI PIDDEGKELK SELTKRWQSL
     751  FPVRATRMFI HYSMQSIKTD EEFKMLQDLW RPRIVPILPY ITRLLYQLQS
     801  YHDPDNWKGL PTVVQSFVKY STIERFWEAG ASNKSKDEFI DEHMKAMQTL
     851  RDFADSVGHI IRYTREYTLL VLSAISSLGS VFYLLDESPD LLLNSIAIFK
     901  PGSNEISPGV STHGWKHIMN IAIRPILKGC PKDCLGKFMP AFLPKLFEIL
     951  DLLLCQKWSS HMNDMDMNPV PTDDDQMTEE ILEENLLRQL TTVVVRIVID
    1001  CVGQGNANPN SAKSRLNNHQ MEMRKIIFND LNTLAPFLKL LNHLISFKDT
    1051  KCSFNSILVM KCCLTSVLNQ NNTVDEYFTF EVMKNLLLNV LCNSAFKDSF
    1101  HEALYAFTVI FLTLCKEYPS ARAFLFEISN GYNIDELYRN LRSVDEYKTQ
    1151  RALMIDFIDW VKSTSGKEDG NVDHAGDERK RQEKREAILK KANERLIKKN
    1201  KENGDMLDDP NIEDGAVGNL FDDN*

external links for Msn5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Msn5p

1)	Akada R, et al. (1996) Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics 143(1):103-17
2)	Blondel M, et al. (1999) Nuclear export of Far1p in response to pheromones requires the export receptor Msn5p/Ste21p. Genes Dev 13(17):2284-300
3)	Kaffman A, et al. (1998) The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus. Nature 396(6710):482-6
4)	Alepuz PM, et al. (1999) The Saccharomyces cerevisiae RanGTP-binding protein msn5p is involved in different signal transduction pathways. Genetics 153(3):1219-31
5)	Yoshida K and Blobel G (2001) The karyopherin Kap142p/Msn5p mediates nuclear import and nuclear export of different cargo proteins. J Cell Biol 152(4):729-40
6)	Queralt E and Igual JC (2003) Cell cycle activation of the Swi6p transcription factor is linked to nucleocytoplasmic shuttling. Mol Cell Biol 23(9):3126-40
7)	Murthi A, et al. (2010) Regulation of tRNA Bidirectional Nuclear-Cytoplasmic Trafficking in Saccharomyces cerevisiae. Mol Biol Cell 21(4):639-49
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41