BFR2/YDR299W Protein Information

Standard Name	Bfr2p 1
Systematic Name	Ydr299wp
ORF Classification	Verified
Description	Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock (2, 3)
Name Description	BreFeldin A Resistance 1

Experimental Data
Molecules/cell	15400 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	534
Molecular Weight (Da)	61,203
Isoelectric Point (pI)	4.51

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bfr2p (InterPro)
Physical Interactions	There are 68 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005300C \| Q06631 MIPS: YDR299W NCBI: 398366449 \| 51830274 \| 74583566 \| 849214 \| NP_010585.3 \| NM_001180607.3 GenBank/EMBL/DDBJ: DAA12138.1 \| AY723789 \| U28374

Amino Acid Sequence (or in FASTA format)
1 MEKSLADQIS DIAIKPVNKD FDIEDEENAS LFQHNEKNGE SDLSDYGNSN 51 TEETKKAHYL EVEKSKLRAE KGLELNDPKY TGVKGSRQAL YEEVSENEDE 101 EEEEEEEEEK EEDALSFRTD SEDEEVEIDE EESDADGGET EEAQQKRHAL 151 SKLIQQETKQ AINKLSQSVQ RDASKGYSIL QQTKLFDNII DLRIKLQKAV 201 IAANKLPLTT ESWEEAKMDD SEETKRLLKE NEKLFNNLFN RLINFRIKFQ 251 LGDHITQNEE VAKHKLSKKR SLKELYQETN SLDSELKEYR TAVLNKWSTK 301 VSSASGNAAL SSNKFKAINL PADVQVENQL SDMSRLMKRT KLNRRNITPL 351 YFQKDCANGR LPELISPVVK DSVDDNENSD DGLDIPKNYD PRRKDNNAID 401 ITENPYVFDD EDFYRVLLND LIDKKISNAH NSESAAITIT STNARSNNKL 451 KKNIDTKASK GRKLNYSVQD PIANYEAPIT SGYKWSDDQI DEFFAGLLGQ 501 RVNFNENEDE EQHARIENDE ELEAVKNDDI QIFG*

Amino Acid Sequence (or in FASTA format)

       1  MEKSLADQIS DIAIKPVNKD FDIEDEENAS LFQHNEKNGE SDLSDYGNSN
      51  TEETKKAHYL EVEKSKLRAE KGLELNDPKY TGVKGSRQAL YEEVSENEDE
     101  EEEEEEEEEK EEDALSFRTD SEDEEVEIDE EESDADGGET EEAQQKRHAL
     151  SKLIQQETKQ AINKLSQSVQ RDASKGYSIL QQTKLFDNII DLRIKLQKAV
     201  IAANKLPLTT ESWEEAKMDD SEETKRLLKE NEKLFNNLFN RLINFRIKFQ
     251  LGDHITQNEE VAKHKLSKKR SLKELYQETN SLDSELKEYR TAVLNKWSTK
     301  VSSASGNAAL SSNKFKAINL PADVQVENQL SDMSRLMKRT KLNRRNITPL
     351  YFQKDCANGR LPELISPVVK DSVDDNENSD DGLDIPKNYD PRRKDNNAID
     401  ITENPYVFDD EDFYRVLLND LIDKKISNAH NSESAAITIT STNARSNNKL
     451  KKNIDTKASK GRKLNYSVQD PIANYEAPIT SGYKWSDDQI DEFFAGLLGQ
     501  RVNFNENEDE EQHARIENDE ELEAVKNDDI QIFG*

external links for Bfr2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Bfr2p

1)	Chabane S, et al. (1998) Over-expression of the yeast BFR2 gene partially suppresses the growth defects induced by Brefeldin A and by four ER-to-Golgi mutations. Curr Genet 33(1):21-8
2)	Chabane S and Kepes F (1998) Expression of the yeast BFR2 gene is regulated at the transcriptional level and through degradation of its product. Mol Gen Genet 258(3):215-21
3)	Bernstein KA, et al. (2004) The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell 3(6):1619-26
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41