GCN2/YDR283C Protein Information

Standard Name	Gcn2p
Systematic Name	Ydr283cp
Alias	Aas1p 1 , Ndr2p 2 , Aas102p 2
ORF Classification	Verified
Description	Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control (3, 4, 5, 6)
Name Description	General Control Nonderepressible 7

Experimental Data
Molecules/cell	279 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,659
Molecular Weight (Da)	190,192
Isoelectric Point (pI)	6.31

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gcn2p (InterPro)
Physical Interactions	There are 94 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053218 \| P15442 MIPS: YDR283C NCBI: 114152808 \| 121064 \| 1332634 \| 171578 \| 172183 \| 187609190 \| 398366405 \| 71042541 \| 71042542 \| 71042543 \| 71042544 \| 71042545 \| 71042546 \| 71042556 \| 71042557 \| 71042558 \| 71042559 \| 71042560 \| 71042561 \| 71042562 \| 71042563 \| 71042564 \| 71042565 \| NP_010569.3 \| NM_001180591.3 GenBank/EMBL/DDBJ: DAA12123.1 \| M20487 \| M27082 \| U51030
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MSLSHLTLDQ YYEIQCNELE AIRSIYMDDF TDLTKRKSSW DKQPQIIFEI 51 TLRSVDKEPV ESSITLHFAM TPMYPYTAPE IEFKNVQNVM DSQLQMLKSE 101 FKKIHNTSRG QEIIFEITSF TQEKLDEFQN VVNTQSLEDD RLQRIKETKE 151 QLEKEEREKQ QETIKKRSDE QRRIDEIVQR ELEKRQDDDD DLLFNRTTQL 201 DLQPPSEWVA SGEAIVFSKT IKAKLPNNSM FKFKAVVNPK PIKLTSDIFS 251 FSKQFLVKPY IPPESPLADF LMSSEMMENF YYLLSEIELD NSYFNTSNGK 301 KEIANLEKEL ETVLKAKHDN VNRLFGYTVE RMGRNNATFV WKIRLLTEYC 351 NYYPLGDLIQ SVGFVNLATA RIWMIRLLEG LEAIHKLGIV HKCINLETVI 401 LVKDADFGST IPKLVHSTYG YTVLNMLSRY PNKNGSSVEL SPSTWIAPEL 451 LKFNNAKPQR LTDIWQLGVL FIQIISGSDI VMNFETPQEF LDSTSMDETL 501 YDLLSKMLNN DPKKRLGTLE LLPMKFLRTN IDSTINRFNL VSESVNSNSL 551 ELTPGDTITV RGNGGRTLSQ SSIRRRSFNV GSRFSSINPA TRSRYASDFE 601 EIAVLGQGAF GQVVKARNAL DSRYYAIKKI RHTEEKLSTI LSEVMLLASL 651 NHQYVVRYYA AWLEEDSMDE NVFESTDEES DLSESSSDFE ENDLLDQSSI 701 FKNRTNHDLD NSNWDFISGS GYPDIVFENS SRDDENEDLD HDTSSTSSSE 751 SQDDTDKESK SIQNVPRRRN FVKPMTAVKK KSTLFIQMEY CENRTLYDLI 801 HSENLNQQRD EYWRLFRQIL EALSYIHSQG IIHRDLKPMN IFIDESRNVK 851 IGDFGLAKNV HRSLDILKLD SQNLPGSSDN LTSAIGTAMY VATEVLDGTG 901 HYNEKIDMYS LGIIFFEMIY PFSTGMERVN ILKKLRSVSI EFPPDFDDNK 951 MKVEKKIIRL LIDHDPNKRP GARTLLNSGW LPVKHQDEVI KEALKSLSNP 1001 SSPWQQQVRE SLFNQSYSLT NDILFDNSVP TSTPFANILR SQMTEEVVKI 1051 FRKHGGIENN APPRIFPKAP IYGTQNVYEV LDKGGTVLQL QYDLTYPMAR 1101 YLSKNPSLIS KQYRMQHVYR PPDHSRSSLE PRKFGEIDFD IISKSSSESG 1151 FYDAESLKII DEILTVFPVF EKTNTFFILN HADILESVFN FTNIDKAQRP 1201 LVSRMLSQVG FARSFKEVKN ELKAQLNISS TALNDLELFD FRLDFEAAKK 1251 RLYKLMIDSP HLKKIEDSLS HISKVLSYLK PLEVARNVVI SPLSNYNSAF 1301 YKGGIMFHAV YDDGSSRNMI AAGGRYDTLI SFFARPSGKK SSNTRKAVGF 1351 NLAWETIFGI AQNYFKLASG NRIKKRNRFL KDTAVDWKPS RCDVLISSFS 1401 NSLLDTIGVT ILNTLWKQNI KADMLRDCSS VDDVVTGAQQ DGIDWILLIK 1451 QQAYPLTNHK RKYKPLKIKK LSTNVDIDLD LDEFLTLYQQ ETGNKSLIND 1501 SLTLGDKADE FKRWDENSSA GSSQEGDIDD VVAGSTNNQK VIYVPNMATR 1551 SKKANKREKW VYEDAARNSS NMILHNLSNA PIITVDALRD ETLEIISITS 1601 LAQKEEWLRK VFGSGNNSTP RSFATSIYNN LSKEAHKGNR WAILYCHKTG 1651 KSSVIDLQR*

Amino Acid Sequence (or in FASTA format)

       1  MSLSHLTLDQ YYEIQCNELE AIRSIYMDDF TDLTKRKSSW DKQPQIIFEI
      51  TLRSVDKEPV ESSITLHFAM TPMYPYTAPE IEFKNVQNVM DSQLQMLKSE
     101  FKKIHNTSRG QEIIFEITSF TQEKLDEFQN VVNTQSLEDD RLQRIKETKE
     151  QLEKEEREKQ QETIKKRSDE QRRIDEIVQR ELEKRQDDDD DLLFNRTTQL
     201  DLQPPSEWVA SGEAIVFSKT IKAKLPNNSM FKFKAVVNPK PIKLTSDIFS
     251  FSKQFLVKPY IPPESPLADF LMSSEMMENF YYLLSEIELD NSYFNTSNGK
     301  KEIANLEKEL ETVLKAKHDN VNRLFGYTVE RMGRNNATFV WKIRLLTEYC
     351  NYYPLGDLIQ SVGFVNLATA RIWMIRLLEG LEAIHKLGIV HKCINLETVI
     401  LVKDADFGST IPKLVHSTYG YTVLNMLSRY PNKNGSSVEL SPSTWIAPEL
     451  LKFNNAKPQR LTDIWQLGVL FIQIISGSDI VMNFETPQEF LDSTSMDETL
     501  YDLLSKMLNN DPKKRLGTLE LLPMKFLRTN IDSTINRFNL VSESVNSNSL
     551  ELTPGDTITV RGNGGRTLSQ SSIRRRSFNV GSRFSSINPA TRSRYASDFE
     601  EIAVLGQGAF GQVVKARNAL DSRYYAIKKI RHTEEKLSTI LSEVMLLASL
     651  NHQYVVRYYA AWLEEDSMDE NVFESTDEES DLSESSSDFE ENDLLDQSSI
     701  FKNRTNHDLD NSNWDFISGS GYPDIVFENS SRDDENEDLD HDTSSTSSSE
     751  SQDDTDKESK SIQNVPRRRN FVKPMTAVKK KSTLFIQMEY CENRTLYDLI
     801  HSENLNQQRD EYWRLFRQIL EALSYIHSQG IIHRDLKPMN IFIDESRNVK
     851  IGDFGLAKNV HRSLDILKLD SQNLPGSSDN LTSAIGTAMY VATEVLDGTG
     901  HYNEKIDMYS LGIIFFEMIY PFSTGMERVN ILKKLRSVSI EFPPDFDDNK
     951  MKVEKKIIRL LIDHDPNKRP GARTLLNSGW LPVKHQDEVI KEALKSLSNP
    1001  SSPWQQQVRE SLFNQSYSLT NDILFDNSVP TSTPFANILR SQMTEEVVKI
    1051  FRKHGGIENN APPRIFPKAP IYGTQNVYEV LDKGGTVLQL QYDLTYPMAR
    1101  YLSKNPSLIS KQYRMQHVYR PPDHSRSSLE PRKFGEIDFD IISKSSSESG
    1151  FYDAESLKII DEILTVFPVF EKTNTFFILN HADILESVFN FTNIDKAQRP
    1201  LVSRMLSQVG FARSFKEVKN ELKAQLNISS TALNDLELFD FRLDFEAAKK
    1251  RLYKLMIDSP HLKKIEDSLS HISKVLSYLK PLEVARNVVI SPLSNYNSAF
    1301  YKGGIMFHAV YDDGSSRNMI AAGGRYDTLI SFFARPSGKK SSNTRKAVGF
    1351  NLAWETIFGI AQNYFKLASG NRIKKRNRFL KDTAVDWKPS RCDVLISSFS
    1401  NSLLDTIGVT ILNTLWKQNI KADMLRDCSS VDDVVTGAQQ DGIDWILLIK
    1451  QQAYPLTNHK RKYKPLKIKK LSTNVDIDLD LDEFLTLYQQ ETGNKSLIND
    1501  SLTLGDKADE FKRWDENSSA GSSQEGDIDD VVAGSTNNQK VIYVPNMATR
    1551  SKKANKREKW VYEDAARNSS NMILHNLSNA PIITVDALRD ETLEIISITS
    1601  LAQKEEWLRK VFGSGNNSTP RSFATSIYNN LSKEAHKGNR WAILYCHKTG
    1651  KSSVIDLQR*

external links for Gcn2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Gcn2p

1)	Hinnebusch AG and Fink GR (1983) Positive regulation in the general amino acid control of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 80(17):5374-8
2)	Thireos G, et al. (1984) 5' untranslated sequences are required for the translational control of a yeast regulatory gene. Proc Natl Acad Sci U S A 81(16):5096-100
3)	Kubota H, et al. (2001) Budding yeast GCN1 binds the GI domain to activate the eIF2alpha kinase GCN2. J Biol Chem 276(20):17591-6
4)	Garcia-Barrio M, et al. (2000) Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2alpha kinase GCN2 is required for GCN2 activation. EMBO J 19(8):1887-99
5)	Hinnebusch AG and Natarajan K (2002) Gcn4p, a master regulator of gene expression, is controlled at multiple levels by diverse signals of starvation and stress. Eukaryot Cell 1(1):22-32
6)	Menacho-Marquez M, et al. (2007) Gcn2p regulates a G1/S cell cycle checkpoint in response to DNA damage. Cell Cycle 6(18):2302-5
7)	Lucchini G, et al. (1984) Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol 4(7):1326-33
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41